Long-Term Consumption of Anthocyanin-Rich Fruit Juice: Impact on Gut Microbiota and Antioxidant Markers in Lymphocytes of Healthy Males

被引:14
|
作者
Groh, Isabel Anna Maria [1 ,2 ]
Riva, Alessandra [3 ]
Braun, Dominik [1 ]
Sutherland, Heidi G. [4 ]
Williams, Owen [3 ]
Bakuradze, Tamara [5 ]
Pahlke, Gudrun [1 ]
Richling, Elke [5 ]
Haupt, Larisa M. [4 ]
Griffiths, Lyn R. [4 ]
Berry, David [3 ]
Marko, Doris [1 ]
机构
[1] Univ Vienna, Fac Chem, Dept Food Chem & Toxicol, Waehringerstr 38, A-1090 Vienna, Austria
[2] Univ Hosp Tuebingen, Dept Expt & Clin Pharmacol & Pharmacogenom, Div Pharmacogenom, Wilhelmstr 56, D-72074 Tubingen, Germany
[3] Univ Vienna, Dept Microbiol & Ecosyst Sci, Ctr Microbiol & Environm Syst Sci, Althanstr 14, A-1090 Vienna, Austria
[4] Univ Technol QUT Queensland, Inst Hlth & Biomed Innovat, Sch Biomed Sci, Genom Res Ctr,Ctr Genom & Personalised Hlth, 60 Musk Ave, Kelvin Grove, Qld 4059, Australia
[5] Univ Kaiserslautern, Dept Chem, Food Chem & Toxicol, Erwin Schroedinger Str 52, D-67663 Kaiserslautern, Germany
关键词
anthocyanin; intervention study; nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2); antioxidant effects; microbiota; TRANSCRIPTION FACTOR NRF2; GENE-TRANSCRIPTION; POLYPHENOLS; METABOLISM; DISCOVERY; SEQUENCES; OBESITY; TARGET; COFFEE; DIET;
D O I
10.3390/antiox10010027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polyphenols are considered protective against diseases associated with oxidative stress. Short-term intake of an anthocyanin-rich fruit juice resulted in significantly reduced deoxyribonucleic acid (DNA) strand-breaks in peripheral blood lymphocytes (PBLs) and affected antioxidant markers in healthy volunteers. Consequently, effects of long-term consumption of fruit juice are of particular interest. In focus was the impact on nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2), the Nrf2-regulated genes NAD(P)H quinone oxidoreductase 1 (NQO-1) and heme oxygenase 1 (HO-1) as well as effects on the gut microbiota. In a nine-week placebo-controlled intervention trial with 57 healthy male volunteers, consumption of anthocyanin-rich juice significantly increased NQO-1 and HO-1 transcript levels in PBLs compared to a placebo beverage as measured by real-time polymerase chain reaction (PCR). Three Nrf2-promotor single nucleotide polymorphisms (SNPs), analyzed by pyrosequencing, indicated an association between individual Nrf2 transcript levels and genotype. Moreover, the Nrf2 genotype appeared to correlate with the presence of specific microbial organisms identified by 16S-PCR and classified as Spirochaetaceae. Furthermore, the microbial community was significantly affected by the duration of juice consumption and intake of juice itself. Taken together, long-term consumption of anthocyanin-rich fruit juice affected Nrf2-dependent transcription in PBLs, indicating systemic effects. Individual Nrf2 genotypes may influence the antioxidant response, thus requiring consideration in future intervention studies focusing on the Nrf2 pathway. Anthocyanin-rich fruit juice had an extensive impact on the gut microbiota.
引用
收藏
页码:1 / 20
页数:18
相关论文
共 12 条
  • [1] Consumption of anthocyanin-rich Queen Garnet plum juice reduces platelet activation related thrombogenesis in healthy volunteers
    Santhakumar, Abishek Bommannan
    Kundur, Avinash Reddy
    Fanning, Kent
    Netzel, Michael
    Stanley, Roger
    Singh, Indu
    JOURNAL OF FUNCTIONAL FOODS, 2015, 12 : 11 - 22
  • [2] Effect of Blueberry Anthocyanin-Rich Extracts on Peripheral and Hippocampal Antioxidant Defensiveness: The Analysis of the Serum Fatty Acid Species and Gut Microbiota Profile
    Si, Xu
    Bi, Jinfeng
    Chen, Qinqin
    Cui, Huijun
    Bao, Yiwen
    Tian, Jinlong
    Shu, Chi
    Wang, Yuehua
    Tan, Hui
    Zhang, Weijia
    Chen, Yi
    Li, Bin
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 69 (12) : 3658 - 3666
  • [3] The Impact of Long-Term Intake of Phenolic Compounds-Rich Grape Pomace on Rat Gut Microbiota
    Chacar, Stephanie
    Itani, Tarek
    Hajal, Joelle
    Saliba, Youakim
    Louka, Nicolas
    Faivre, Jean-Francois
    Maroun, Richard
    Fares, Nassim
    JOURNAL OF FOOD SCIENCE, 2018, 83 (01) : 246 - 251
  • [4] Bolus Consumption of a Specifically Designed Fruit Juice Rich in Anthocyanins and Ascorbic Acid Did Not Influence Markers of Antioxidative Defense in Healthy Humans
    Ellinger, Sabine
    Gordon, Andre
    Kuerten, Mira
    Jungfer, Elvira
    Zimmermann, Benno F.
    Zur, Berndt
    Ellinger, Joerg
    Marx, Friedhelm
    Stehle, Peter
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (45) : 11292 - 11300
  • [5] Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins
    P Winkler
    S Ellinger
    A M Boetzer
    B M Arendt
    H K Berthold
    J K Rockstroh
    U Spengler
    R Goerlich
    European Journal of Clinical Nutrition, 2004, 58 : 317 - 325
  • [6] Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins
    Winkler, P
    Ellinger, S
    Boetzer, AM
    Arendt, BM
    Berthold, HK
    Rockstroh, JK
    Spengler, U
    Goerlich, R
    EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2004, 58 (02) : 317 - 325
  • [7] Fecal microbiota and metabolomics revealed the effect of long-term consumption of gallic acid on canine lipid metabolism and gut health
    Yang, Kang
    Jian, Shiyan
    Guo, Dan
    Wen, Chaoyu
    Xin, Zhongquan
    Zhang, Limeng
    Kuang, Tao
    Wen, Jiawei
    Yin, Yulong
    Deng, Baichuan
    FOOD CHEMISTRY-X, 2022, 15
  • [8] Plasma antioxidant capacity of HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate containing antioxidant polyphenols
    Arendt, BM
    Boetzer, AM
    Lemoch, H
    Winkler, P
    Rockstroh, JK
    Berthold, H
    Spengler, U
    Goerlich, R
    EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2001, 55 (09) : 786 - 792
  • [9] Plasma antioxidant capacity of HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit–vegetable-concentrate containing antioxidant polyphenols
    BM Arendt
    AM Boetzer
    H Lemoch
    P Winkler
    JK Rockstroh
    HK Berthold
    U Spengler
    R Goerlich
    European Journal of Clinical Nutrition, 2001, 55 : 786 - 792
  • [10] Impact of Long-Term Proton Pump Inhibitor Therapy on Gut Microbiota in F344 Rats: Pilot Study
    Shin, Cheol Min
    Kim, Nayoung
    Kim, Yong Sung
    Nam, Ryoung Hee
    Park, Ji Hyun
    Lee, Dong Ho
    Seok, Yeong-Jae
    Kim, Yeon-Ran
    Kim, Joo-Hyon
    Kim, Jung Min
    Kim, Joo Sung
    Jung, Hyun Chae
    GUT AND LIVER, 2016, 10 (06) : 896 - 901