Single-molecule force spectroscopy on Bombyx mori silk fibroin by atomic force microscopy

被引：49
|
作者：
Zhang, WK
Xu, QB
Zou, S
Li, HB
Xu, WQ
Zhang, X [1 ]
Shao, ZZ
Kudera, M
Gaub, HE
机构：
[1] Jilin Univ, Key Lab Supramol Struct & Spect, Changchun 130023, Peoples R China
[2] Jilin Univ, Dept Chem, Changchun 130023, Peoples R China
[3] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China
[4] Fudan Univ, Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[5] Univ Munich, Lehrstuhl Angew Phys, D-80799 Munich, Germany
来源：
LANGMUIR | 2000年 / 16卷 / 09期
关键词：
D O I：
10.1021/la991230y
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new atomic force microscopy (AFM)-based technique, single-molecule force spectroscopy (SMFS), was used to study the nanomechanics of Bombyx mori silk fibroin. Three types of force-extension curves were found in the system. A modified freely jointed chain (MFJC) model can fit two of the three types well, but the fit parameters are different. The third type of force curve, in which there exists a plateau, cannot be modeled by MFJC. These results may show that there are three kinds of conformations in the silk fibroin system and SMFS can "distinguish" different conformations of the polymer chains.
引用
收藏
页码：4305 / 4308
页数：4
相关论文
共 50 条
  • [1] Atomic force microscopy:: Bombyx mori silk fibroin molecules and their higher order structure
    Inoue, SI
    Magoshi, J
    Tanaka, T
    Magoshi, Y
    Becker, M
    JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 2000, 38 (11) : 1436 - 1439
  • [2] Segmented nanofibers of spider dragline silk: Atomic force microscopy and single-molecule force spectroscopy
    Oroudjev, E
    Soares, J
    Arcdiacono, S
    Thompson, JB
    Fossey, SA
    Hansma, HG
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 : 6460 - 6465
  • [3] Single-Molecule Atomic Force Microscopy Force Spectroscopy Study of Aβ-40 Interactions
    Kim, Bo-Hyun
    Palermo, Nicholas Y.
    Lovas, Sandor
    Zaikova, Tatiana
    Keana, John F. W.
    Lyubchenko, Yuri L.
    BIOCHEMISTRY, 2011, 50 (23) : 5154 - 5162
  • [4] Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
    Neuman, Keir C.
    Nagy, Attila
    NATURE METHODS, 2008, 5 (06) : 491 - 505
  • [5] Single-molecule force spectroscopy: Optical tweezers, magnetic tweezers and atomic force microscopy
    Neuman K.C.
    Nagy A.
    Nature Methods, 2008, 5 (6) : 491 - 505
  • [6] Single molecule force spectroscopy on polysaccharides by atomic force microscopy
    Rief, M
    Oesterhelt, F
    Heymann, B
    Gaub, HE
    SCIENCE, 1997, 275 (5304) : 1295 - 1297
  • [7] Single molecule atomic force microscopy and force spectroscopy of chitosan
    Kocun, Marta
    Grandbois, Michel
    Cuccia, Louis A.
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2011, 82 (02) : 470 - 476
  • [8] Going Vertical To Improve the Accuracy of Atomic Force Microscopy Based Single-Molecule Force Spectroscopy
    Walder, Robert
    Van Patten, William J.
    Adhikari, Ayush
    Perkins, Thomas T.
    ACS NANO, 2018, 12 (01) : 198 - 207
  • [9] Correction of systematic errors in single-molecule force spectroscopy with polymeric tethers by atomic force microscopy
    Ray, Chad
    Brown, Jason R.
    Akhremitchev, Boris B.
    JOURNAL OF PHYSICAL CHEMISTRY B, 2007, 111 (08): : 1963 - 1974
  • [10] The structure and function of cell membranes examined by atomic force microscopy and single-molecule force spectroscopy
    Shan, Yuping
    Wang, Hongda
    CHEMICAL SOCIETY REVIEWS, 2015, 44 (11) : 3617 - 3638
12345