A study of metal concentrations and metallothionein binding capacity in liver, kidney and brain tissues of three Arctic seal species

Arctic seals are known to accumulate relatively high concentrations of potential toxic heavy metals in their vital organs. such as livers and kidneys, as well as in their central nervous system. We therefore decided to determine whether mercury, copper, cadmium and zinc levels in liver, kidney and brain tissues of three Arctic seal species were associated with the intracellular metal-binding protein metallothionein (MT) as a sign of toxic exposure. Samples from four ringed (Phoca hispida), five harp (P. groenlandica) and five hooded (Cystophona cristata) seals taken during field trips to Central West Greenland (Godhavn) and the Barents Sea in the spring of 1999 were used for the present study. In all three seal species concentrations of mercury. zinc and copper were highest in the liver, except for cadmium which was highest in the kidneys. Metal concentrations increased significantly in the order: ringed seal < harp seal <hooded seal for both kidney and liver tissues. MT concentrations were highest in the kidneys and the concentrations increased in the order: ringed seal < hooded seal < harp seal. MIT metal-binding capacity was highest in the kidneys for all three species and increased in the same order: ringed seals (2-10%) < hooded seals (8-15%) < harp seals (27-63%). We therefore suggest that there are species-specific differences in the sub-cellular handling of heavy metals which indicate differences in sensitivity and health implications. However, a larger sample size is needed in order to test the relationship between metal concentrations and MT up-regulation in order to decide which metals are the most important and to elucidate whether the MT binding capacity is sufficient to protect tissues (i.e. kidney) from metal toxicosis. MT with its binding capacity could be a useful marker for environmental exposure to metals and their potential toxicity in the Arctic. (C) 2009 Elsevier B.V. All rights reserved.