Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

被引：6

作者：

Echeverría, MG ^{[1
]}

Nosetto, EO ^{[1
]}

Etcheverrigaray, ME ^{[1
]}

机构：

[1] Natl Univ La Plata, Fac Vet Sci, RA-1900 La Plata, Argentina

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2000年 / 12卷 / 03期

关键词：

D O I：

10.1177/104063870001200313

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions. A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.

引用

页码：266 / 268

页数：3

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