Repression of nitrate uptake by replacement of Asp105 by asparagine in AtNRT3.1 in Arabidopsis thaliana L.

被引：19

作者：

Kawachi, Tahei ^{[1
]}

Sunaga, Yoshihito ^{[1
]}

Ebato, Munehiro ^{[1
]}

Hatanaka, Tetsuya ^{[1
]}

Harada, Hisatomi ^{[1
]}

机构：

[1] Natl Inst Livestock & Grassland Sci, Nasushiobara, Tochigi 3292793, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2006年 / 47卷 / 10期

关键词：

Arabidopsis thaliana; AtNRT3.1; nitrate uptake; single nucleotide mutation;

D O I：

10.1093/pcp/pcl010

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An Arabidopsis mutant (rnc1) with a mutation at the 313th nucleotide from the translational start site of AtNRT3.1 was isolated. The mutation resulted in the replacement of aspartate by asparagine at the 105th amino acid in a region conserved among higher plants. In the rnc1 mutant, both the nitrate concentrations in plants and the nitrate uptake from the medium were < 13% compared with those of the wild type, while AtNRT3.1 mRNA was accumulated similarly and both AtNRT1.1 and AtNRT2.1 mRNA were decreased. These results suggest that the replacement of Asp105 in AtNRT3.1 markedly reduces nitrate uptake and accumulation.

引用

页码：1437 / 1441

页数：5

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