A Bath Challenge Method with Tenacibaculum maritimum for Japanese Flounder Paralichthys olivaceus

被引:10
作者
Nishioka, Toyohiro [1 ]
Watanabe, Ken-ichi [1 ]
Sano, Motohiko [1 ]
机构
[1] Natl Res Inst Aquaculture, Oita 8792602, Japan
来源
FISH PATHOLOGY | 2009年 / 44卷 / 04期
关键词
Tenacibaculum maritimum; gliding bacterial disease; bath challenge; Paralichthys olivaceus; Japanese flounder; DISEASED CULTURED FLOUNDER; FLEXIBACTER-MARITIMUS; MARINE FISHES; INFECTION; BACTERIA; IDENTIFICATION; FLAVOBACTERIUM; COLUMNARIS; STRAINS;
D O I
10.3147/jsfp.44.178
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A bath challenge method with Tenacibaculum maritimum was examined using juvenile Japanese flounder Paralichthys olivaceus. After immersed in the bacterial suspensions at different concentrations for 1 h, fish were maintained at 15 degrees C, 20 degrees C and 25 degrees C. At any temperatures, mortalities were 80-100% and 20-40% in fish challenged at 10(7.9) and 10(6.9) CFU/mL, respectively. No disease signs were observed in fish challenged at 10(5.9) CFU/mL. The affected fish exhibited mouth reddening and skin erosion, and T maritimum were re-isolated from the hemorrhagic skin lesions. The seed lot method used in the present study probably provided reproducible results with high mortality.
引用
收藏
页码:178 / 181
页数:4
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