Fluorescent sensors based on [12]aneN3-modified BODIPY: Discrimination of different biological thiols in aqueous solution and living cells

被引：18

作者：

Gao, Yong-Guang ^{[1
]}

Zhang, Ying ^{[1
]}

Shi, You-Di ^{[1
]}

Hao, Hu-Jun ^{[1
]}

Gong, Bing ^{[1
]}

Lu, Zhong-Lin ^{[1
]}

机构：

[1] Beijing Normal Univ, Coll Chem, Minist Educ, Key Lab Theoret & Computat Photochem, Xinjiekouwai St 19, Beijing 100875, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2016年 / 24卷 / 07期

关键词：

BODIPY; 12]aneN(3); Thiols; Sensor; HIGHLY SELECTIVE DETECTION; GLUTATHIONE DEFICIENCY; RISK FACTOR; CYSTEINE; HOMOCYSTEINE; PROBE; LUMINESCENT; GSH;

D O I：

10.1016/j.bmc.2016.02.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two fluorescent probes, 1 and 2, derived from borondipyrromethene (BODIPY) modified with macrocyclic polyamine [12]aneN(3), were synthesized and applied in the discrimination of cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) with absorption and fluorescent spectroscopy in comparison. It was found that Boc-protected 1 showed highly sensitive and selective recognition of GSH over Cys and Hcy; while probe 2 was able to distinguish the three different thiols due to their different reactivities. With its water-solubility, rapid responsiveness, high sensitivity and low cytotoxicity, probe 2 was successfully applied in the fast detection of three biothiols in living cells. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1550 / 1559

页数：10

共 40 条

[1] Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse [J].