Experimental aspects of biological X-ray absorption spectroscopy

被引：52
作者：
Ascone, I
Meyer-Klaucke, W
Murphy, L
机构：
[1] Univ Paris 11, LURE, F-91898 Orsay, France
[2] DESY, EMBL, D-22607 Hamburg, Germany
[3] Daresbury Lab, Warrington WA4 4AD, Cheshire, England
来源：
JOURNAL OF SYNCHROTRON RADIATION | 2003年 / 10卷
关键词：
BioXAS; metalloproteins; experimental set-up; macromolecules;
D O I：
10.1107/S0909049502022598
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
Spectroscopic techniques, like X-ray absorption spectroscopy, will provide important input for integrated biological projects in genomics and proteomics. This contribution summarizes technical requirements and typical set-ups for both simple and complex biological XAS experiments. An overview on different strategies for sample preparation is discussed in detail. Present and future BioXAS spectrometers are presented to help potential users in locating the spectrometer required for their biological application.
引用
收藏
页码：16 / 22
页数：7
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