Expression and characterization of the human rpS3 in a methylotrophic yeast Pichia pastoris

被引：0

作者：

Lee, JY

Jung, SO

Youn, B

Kwon, OS

Kim, J

机构：

[1] Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea

[2] Mokpo Natl Univ, Coll Nat Sci, Dept Biol, Muan 534729, Chonnam, South Korea

[3] Korea Univ, Dept Biol, Seoul 136701, South Korea

[4] Korea Inst Sci & Technol, Seoul 136791, South Korea

[5] Keimyung Univ, Coll Nat Sci, Dept Microbiol, Taegu 704701, South Korea

来源：

JOURNAL OF MICROBIOLOGY | 2000年 / 38卷 / 02期

关键词：

rpS3; Pichia pastoris; endonuclease activity; nick circle assay;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A human ribosomal protein S3 (rpS3), which also functions as a DNA repair enzyme (UV endonuclease III), was expressed in a methylotrophic yeast, Pichia pastoris, and biochemically characterized. UV endonuclease activity was previously characterized, and this activity of mammalian rpS3 was found to be non-specific upon purification and storage. Under the Pichia expression system, the subcloned rDNA of the human rpS3 gene revealed a peptide of 42 kDa by SDS-PAGE and Western blot, The secreted form of human rpS3 rendered no endonuclease activity while the intracellular form showed UV specific endonuclease activity by the nick circle assay.

引用

页码：88 / 92

页数：5

共 12 条

[1] Heterologous protein expression in the methylotrophic yeast Pichia pastoris [J].