Characterization of triple reassortant H1N1 influenza A viruses from swine in Ohio

被引：34

作者：

Yassine, H. M. ^{[1
]}

Khatri, M. ^{[1
]}

Zhang, Y. J. ^{[2
]}

Lee, C. W. ^{[1
]}

Byrum, B. A. ^{[2
]}

O'Quin, J. ^{[3
]}

Smith, K. A. ^{[3
]}

Saif, Y. M. ^{[1
]}

机构：

[1] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Wooster, OH 44691 USA

[2] Ohio Dept Agr, Anim Dis Diagnost Lab, Reynoldsburg, OH 43068 USA

[3] Ohio Dept Hlth, Reynoldsburg, OH 43068 USA

来源：

VETERINARY MICROBIOLOGY | 2009年 / 139卷 / 1-2期

关键词：

Influenza A viruses; H1N1; Triple reassortants; RECEPTOR-BINDING PROPERTIES; GENETIC-CHARACTERIZATION; HEMAGGLUTININ; PIGS; EVOLUTION; INFECTION; MICE; H5N1; SPECIFICITY; PROTECTION;

D O I：

10.1016/j.vetmic.2009.04.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An H1N1 influenza A virus, A/swine/Ohio/24366/07, was isolated from pigs in an Ohio county fair. Twenty-six people who came in contact with the infected pigs developed respiratory disease and two of these people were laboratory confirmed as H1N1 by the Centers for Disease Control and Prevention (CDC). The A/swine/Ohio/24366/07 virus we isolated from swine was shown at the CDC to have 100% identical genome sequence to the human virus associated with the county fair. This prompted us to characterize three swine and two human origin H1N1 influenza A viruses isolated at different time points in the State of Ohio. The three swine viruses were shown to be triple reassortant viruses harboring genes of human (PB1), swine (HA, NA, NP, M, and NS), and avian (PB2 and PA) lineage viruses. Although viruses evaluated in this study were isolated during a short time interval (3 years), genetic drift was observed within the HA and NA genes, including changes at the receptor binding and antigenic sites of HA1 protein. Nevertheless, all viruses exhibited antigenic similarity as evaluated with hemagglutination inhibition and virus neutralizing tests. Internal genes were similar to other reassortant viruses of various subtypes currently circulating in the United States. Interestingly, two of the swine viruses including the 2007 isolate replicated well in human airway epithelial cells, however, another virus isolated in 2006 showed very little replication. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：132 / 139

页数：8

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