Biodiesel production in a magnetically-stabilized, fluidized bed reactor with an immobilized lipase in magnetic chitosan microspheres

被引：49

作者：

Zhou, Gui-xiong ^{[1
]}

Chen, Guan-yi ^{[1
]}

Yan, Bei-bei ^{[1
]}

机构：

[1] Tianjin Univ, State Key Lab Engines, Sch Environm Sci & Technol, Tianjin 300072, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2014年 / 36卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Biodiesel; Magnetic chitosan microspheres; Magnetically stabilized fluidized bed reactor; Transesterification; FUEL;

D O I：

10.1007/s10529-013-1336-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biodiesel production by immobilized Rhizopus oryzae lipase in magnetic chitosan microspheres (MCMs) was carried out using soybean oil and methanol in a magnetically-stabilized, fluidized bed reactor (MSFBR). The maximum content of methyl ester in the reaction mixture reached 91.3 (w/v) at a fluid flow rate of 25 ml/min and a magnetic field intensity of 150 Oe. In addition, the MCMs-immobilized lipase in the reactor showed excellent reusability, retaining 82 % productivity even after six batches, which was much better than that in a conventional fluidized bed reactor. These results suggested that a MSFRB using MCMs-immobilized lipase is a promising method for biodiesel production.

引用

页码：63 / 68

页数：6

共 13 条

[1] Performance of immobilized glucoamylase in a magnetically stabilized fluidized bed reactor (MSFBR) [J].

Bahar, T ;

Çelebi, SS .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 26 (01) :28-33

[2] Immobilizing enzymes: How to create more suitable biocatalysts [J].

Bornscheuer, UT .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (29) :3336-3337

[3] Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles [J].

Chan, H. T. ;

Do, Y. Y. ;

Huang, P. L. ;

Chien, P. L. ;

Chan, T. S. ;

Liu, R. S. ;

Huang, C. Y. ;

Yang, S. Y. ;

Horng, H. E. .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2006, 304 (01) :E415-E417

[4] Enzymatic conversion of waste cooking oils into alternative fuel-biodiesel [J].

Chen, Guanyi ;

Ying, Ming ;

Li, Weizhun .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 132 (1-3) :911-921

[5]

Du W, 2007, GREEN CHEM, V9, P173, DOI 10.1039/B613704K

[6] Candida rugosa Lipase Supported on High Crystallinity Chitosan as Biocatalyst for the Synthesis of 1-Butyl Oleate [J].

Eduardo Orrego, Carlos ;

Sigifredo Valencia, Jesus ;

Zapata, Catalina .

CATALYSIS LETTERS, 2009, 129 (3-4) :312-322

[7] Biodiesel fuel production by transesterification of oils [J].

Fukuda, H. (fukuda@cx.kobe-u.ac.jp), 2001, Society of Fermentation and Bioengineering, Japan (92)

[8] RETRACTED: Optimization of Biodiesel Production Using a Magnetically Stabilized Fluidized Bed Reactor (Retracted article. See vol. 55, pg. 561, 2013) [J].

Guo, Pingmei ;

Huang, Fenghong ;

Huang, Qingde ;

Zheng, Chang .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2012, 89 (03) :497-504

[9] Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate [J].

Hu, Bin ;

Pan, Jiang ;

Yu, Hui-Lei ;

Liu, Jian-Wen ;

Xu, Jian-He .

PROCESS BIOCHEMISTRY, 2009, 44 (09) :1019-1024

[10] Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase [J].

Iso, M ;

Chen, BX ;

Eguchi, M ;

Kudo, T ;

Shrestha, S .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 16 (01) :53-58

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