Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging

被引：66

作者：

Dilipkumar, Ashwathama ^{[1
,2
]}

Al-Shemmary, Alaa ^{[1
,2
]}

Kreiss, Lucas ^{[1
,2
]}

Cvecek, Kristian ^{[2
,3
]}

Carle, Birgitta ^{[1
]}

Knieling, Ferdinand ^{[4
,5
]}

Menezes, Jean Gonzales ^{[4
]}

Thoma, Oana-Maria ^{[2
,4
]}

Schmidt, Michael ^{[2
,3
]}

Neurath, Markus F. ^{[2
,4
]}

Waldner, Maximilian ^{[2
,4
]}

Friedrich, Oliver ^{[1
,2
]}

Schuermann, Sebastian ^{[1
,2
]}

机构：

[1] Friedrich Alexander Univ Erlangen Nurnberg FAU, Inst Med Biotechnol, Paul Gordan Str 3, D-91052 Erlangen, Germany

[2] Friedrich Alexander Univ Erlangen Nurnberg FAU, Erlangen Grad Sch Adv Opt Technol, Paul Gordan Str 7, D-91052 Erlangen, Germany

[3] Friedrich Alexander Univ Erlangen Nurnberg FAU, Inst Photon Technol, Konrad Zuse Str 3-5, D-91052 Erlangen, Germany

[4] Univ Hosp Erlangen, Dept Internal Med 1, Ulmenweg 18, D-91054 Erlangen, Germany

[5] Univ Hosp Erlangen, Dept Pediat & Adolescent Med, Loschgestr 15, D-91054 Erlangen, Germany

来源：

ADVANCED SCIENCE | 2019年 / 6卷 / 08期

关键词：

endoscopy; inflammatory bowel disease; multiphoton microscopy; optical imaging; COLON-CANCER; TOMOGRAPHY; ENDOSCOPY; COLITIS; VIEW; MICE;

D O I：

10.1002/advs.201801735

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label-free in vivo imaging in small animals via side viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period often days, demonstrating the capabilities of label-free endomicroscopy for longitudinal studies for the first time.

引用

页数：8

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