Surface plasmon resonance biosensor for genetically modified organisms detection

被引：92

作者：

Mariotti, E ^{[1
]}

Minunni, M ^{[1
]}

Mascini, M ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Chim, I-50121 Florence, Italy

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 453卷 / 02期

关键词：

surface plasmon resonance; biosensor; polymerase chain reaction;

D O I：

10.1016/S0003-2670(01)01458-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of a surface plasmon resonance (SPR) affinity biosensor based on DNA hybridisation is described. This biosensor has been applied to genetically modified organisms (GMOs) detection. Single stranded DNA (ssDNA) probes were immobilised on the sensor chip of an SPR device and the hybridisation between the immobilised probe and the complementary sequence (target) was monitored. The probe sequences were internal to the sequence of 35S promoter and NOS terminator which are inserted sequences in the genome of GMO regulating the transgene expression. The system has been optimised using synthetic oligonucleotides, then applied to real samples analysis. Samples, containing the transgenic target sequences, were amplified by polymerase chain reaction (PCR) and then detected with the SPR biosensor. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：165 / 172

页数：8

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