Long Noncoding RNA EBF3-AS Promotes Neuron Apoptosis in Alzheimer's Disease

被引：89

作者：

Gu, Cheng ^{[1
]}

Chen, Cheng ^{[2
]}

Wu, Ruipeng ^{[1
]}

Dong, Tong ^{[1
]}

Hu, Xiaojuan ^{[1
]}

Yao, Yuping ^{[1
]}

Zhang, Yi ^{[1
]}

机构：

[1] Gansu Prov Hosp, Dept Neurol, 204 West Donggang Rd, Lanzhou 730000, Gansu, Peoples R China

[2] Lanzhou Univ, Dept Galactophore, Hosp 1, Lanzhou, Gansu, Peoples R China

来源：

DNA AND CELL BIOLOGY | 2018年 / 37卷 / 03期

关键词：

Alzheimer's disease; lncRNA EBF3-AS; SH-SY5Y; neurons apoptosis; EXPRESSION;

D O I：

10.1089/dna.2017.4012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alzheimer's disease (AD) is the most common form of dementia; its pathophysiological mechanism remains unclear. Long noncoding RNAs (lncRNAs) play key roles in AD. lncRNA EBF3-AS has been found dysregulated in AD, which is abundantly expressed in the brain. The aim of this study was to investigate the role of EBF3-AS in AD. Results showed that the expressions of lncRNA EBF3-AS and EBF3 (early B cell factor 3) were upregulated in hippocampus of APP/PS1 mice (AD model mice). EBF3-AS knockdown by siRNA inhibited the apoptosis induced by A(25-35) and okadaic acid (OA) in SH-SY5Y. The expression of EBF3 was downregulated in A(25-35)- and OA-treated SH-SY5Y, which was reversed by EBF3-AS knockdown. EBF3 knockdown can reverse the A(25-35)-induced apoptosis in SH-SY5Y. These results revealed that lncRNA EBF3-AS promoted neuron apoptosis in AD, and involved in regulating EBF3 expression. EBF3-AS may be a new therapeutic target for treatment of AD.

引用

页码：220 / 226

页数：7

共 20 条

[1] Targeting noncoding RNAs in disease [J].

Adams, Brian D. ;

Parsons, Christine ;

Walker, Lisa ;

Zhang, Wen Cai ;

Slack, Frank J. .

JOURNAL OF CLINICAL INVESTIGATION, 2017, 127 (03) :761-771

[2]

Alzheimers Association, 2015, Alzheimers Dement, V11, P332

[3] Investigation of 15 of the top candidate genes for late-onset Alzheimer's disease [J].

Belbin, Olivia ;

Carrasquillo, Minerva M. ;

Crump, Michael ;

Culley, Oliver J. ;

Hunter, Talisha A. ;

Ma, Li ;

Bisceglio, Gina ;

Zou, Fanggeng ;

Allen, Mariet ;

Dickson, Dennis W. ;

Graff-Radford, Neill R. ;

Petersen, Ronald C. ;

Morgan, Kevin ;

Younkin, Steven G. .

HUMAN GENETICS, 2011, 129 (03) :273-282

[4] Long Noncoding RNA and Cancer: A New Paradigm [J].

Bhan, Arunoday ;

Soleimani, Milad ;

Mandal, Subhrangsu S. .

CANCER RESEARCH, 2017, 77 (15) :3965-3981

[5] LncRNAs: macromolecules with big roles in neurobiology and neurological diseases [J].

Chen, Ye ;

Zhou, Jun .

METABOLIC BRAIN DISEASE, 2017, 32 (02) :281-291

[6] An intronic ncRNA-dependent regulation of SORL1 expression affecting Aβ formation is upregulated in post-mortem Alzheimer's disease brain samples [J].

Ciarlo, Eleonora ;

Massone, Sara ;

Penna, Ilaria ;

Nizzari, Mario ;

Gigoni, Arianna ;

Dieci, Giorgio ;

Russo, Claudio ;

Florio, Tullio ;

Cancedda, Ranieri ;

Pagano, Aldo .

DISEASE MODELS & MECHANISMS, 2013, 6 (02) :424-433

[7] Drug candidates in clinical trials for Alzheimer's disease [J].

Hung, Shih-Ya ;

Fu, Wen-Mei .

JOURNAL OF BIOMEDICAL SCIENCE, 2017, 24

[8] Molecular and Cellular Mechanism of Okadaic Acid (OKA)-Induced Neurotoxicity: A Novel Tool for Alzheimer's Disease Therapeutic Application [J].

Kamat, Pradip K. ;

Rai, Shivika ;

Swarnkar, Supriya ;

Shukla, Rakesh ;

Nath, Chandishwar .

MOLECULAR NEUROBIOLOGY, 2014, 50 (03) :852-865

[9] Long noncoding RNAs and Alzheimer's disease [J].

Luo, Qiong ;

Chen, Yinghui .

CLINICAL INTERVENTIONS IN AGING, 2016, 11 :867-872

[10] Transcriptomics Profiling of Alzheimer's Disease Reveal Neurovascular Defects, Altered Amyloid-β Homeostasis, and Deregulated Expression of Long Noncoding RNAs [J].

Magistri, Marco ;

Velmeshev, Dmitry ;

Makhmutova, Madina ;

Faghihi, Mohammad Ali .

JOURNAL OF ALZHEIMERS DISEASE, 2015, 48 (03) :647-665

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