Chaperone-Like Activity of β-Casein and Thermal Stability of Alcohol Dehydrogenase

被引：4

作者：

Zakharchenko, N. L. ^{[1
]}

Konnova, T. A. ^{[1
]}

Gogoleva, N. E. ^{[1
]}

Faizullin, D. A. ^{[1
]}

Haertle, T. ^{[2
]}

Zuev, Yu. F. ^{[1
]}

机构：

[1] Russian Acad Sci, Kazan Sci Ctr, Kazan Inst Biochem & Biophys, Kazan 420111, Russia

[2] Inst Natl Rech Agronom Nantes, F-44316 Nantes, France

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2012年 / 38卷 / 02期

关键词：

beta-casein; mutant forms; physico-chemical properties; chaperon-like activity; PROTEIN SECONDARY STRUCTURE;

D O I：

10.1134/S1068162012020136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Correlation between structural peculiarities of beta-casein and its chaperon-like activity was investigated using the recombinant forms of the protein containing the cysteine residues in the polypeptide chain. Aggregation of native and modified forms of beta-casein was studied, as well as their chaperon-like activity towards alcohol dehydrogenase thermal aggregation. It has been shown that dimeric and oligomeric forms of beta-casein, which are formed due to intermolecular disulfide bonds, significantly differ in their physico-chemical and chaperon-like properties from monomeric forms. The thermal stability of alcohol dehydrogenase has been found to depend on the beta-casein concentration.

引用

页码：192 / 197

页数：6

共 20 条

[11] 3-DIMENSIONAL MOLECULAR MODELING OF BOVINE CASEINS - AN ENERGY-MINIMIZED BETA-CASEIN STRUCTURE [J].

KUMOSINSKI, TF ;

BROWN, EM ;

FARRELL, HM .

JOURNAL OF DAIRY SCIENCE, 1993, 76 (04) :931-945

[12]

Kurganov BI, 2002, USPEKHI BIOLOGICH HI, V42, P89

[13] A study of β-casein tertiary structure by intramolecular crosslinking and mass spectrometry [J].

Livney, YD ;

Schwan, AL ;

Dalgleish, DG .

JOURNAL OF DAIRY SCIENCE, 2004, 87 (11) :3638-3647

[14] Thermodynamics of micellization of bovine β-casein studied by high-sensitivity differential scanning calorimetry [J].

Mikheeva, LM ;

Grinberg, NV ;

Grinberg, VY ;

Khokhlov, AR ;

de Kruif, CG .

LANGMUIR, 2003, 19 (07) :2913-2921

[15] Casein proteins as molecular chaperones [J].

Morgan, PE ;

Treweek, TM ;

Lindner, RA ;

Price, WE ;

Carver, JA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (07) :2670-2683

[16] Association behavior of β-casein [J].

O'Connell, JE ;

Grinberg, VY ;

de Kruif, CG .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2003, 258 (01) :33-39

[17] ESTIMATION OF GLOBULAR PROTEIN SECONDARY STRUCTURE FROM CIRCULAR-DICHROISM [J].

PROVENCHER, SW ;

GLOCKNER, J .

BIOCHEMISTRY, 1981, 20 (01) :33-37

[18] Intracellular protein unfolding and aggregation: The role of small heat-shock chaperone proteins [J].

Treweek, TM ;

Morris, AM ;

Carver, JA .

AUSTRALIAN JOURNAL OF CHEMISTRY, 2003, 56 (05) :357-367

[19] Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases [J].

Whitmore, Lee ;

Wallace, B. A. .

BIOPOLYMERS, 2008, 89 (05) :392-400

[20] Chaperone-Like Activities of Different Molecular Forms of β-casein. Importance of Polarity of N-Terminal Hydrophilic Domain [J].

Yousefi, Reza ;

Shchutskaya, Yulia Y. ;

Zimny, Jaroslaw ;

Gaudin, Jean-Charles ;

Moosavi-Movahedi, Ali A. ;

Muronetz, Vladimir I. ;

Zuev, Yuriy F. ;

Chobert, Jean-Marc ;

Haertle, Thomas .

BIOPOLYMERS, 2009, 91 (08) :623-632

← 1 2 →