Transcriptional interference between convergent promoters caused by elongation over the promoter

被引:135
作者
Callen, BP [1 ]
Shearwin, KE [1 ]
Egan, JB [1 ]
机构
[1] Univ Adelaide, Sch Mol & Biomed Sci Biochem, Adelaide, SA 5005, Australia
关键词
D O I
10.1016/j.molcel.2004.05.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcriptional interference with convergent transcription from face-to-face promoters is a potentially important form of gene regulation in all organisms. Using LacZ reporter studies, the mechanism of interference was determined for a pair of face-to-face prokaryotic promoters in which a strong promoter interferes 5.6-fold with a weak promoter, 62 bp away. The promoters were variously rearranged to test different models of interference. Terminating transcription from the strong promoter before it reached the weak promoter dramatically reduced interference, indicating a requirement for the passage of the converging RNAP over the weak promoter. Based on in vitro experiments showing a slow rate of escape for open complexes at the weak promoter and their sensitivity to head-on collisions with elongating RNAP, a sitting duck" model of interference is proposed and supported with in vivo permanganate footprinting. The model is further supported by the analysis of a second set of prokaryotic face-to-face promoters."
引用
收藏
页码:647 / 656
页数:10
相关论文
共 33 条
[1]   PROMOTER OCCLUSION - TRANSCRIPTION THROUGH A PROMOTER MAY INHIBIT ITS ACTIVITY [J].
ADHYA, S ;
GOTTESMAN, M .
CELL, 1982, 29 (03) :939-944
[2]  
CALLEN BP, 2003, THESIS U ADELAIDE AD
[3]   Action at a distance in Cl repressor regulation of the bacteriophage 186 genetic switch [J].
Dodd, IB ;
Egan, JB .
MOLECULAR MICROBIOLOGY, 2002, 45 (03) :697-710
[4]   Octamerization of λ CI repressor is needed for effective repression of PRM and efficient switching from lysogeny [J].
Dodd, IB ;
Perkins, AJ ;
Tsemitsidis, D ;
Egan, JB .
GENES & DEVELOPMENT, 2001, 15 (22) :3013-3022
[5]   CONTROL OF GENE-EXPRESSION IN THE TEMPERATE COLIPHAGE-186 .8. CONTROL OF LYSIS AND LYSOGENY BY A TRANSCRIPTIONAL SWITCH INVOLVING FACE-TO-FACE PROMOTERS [J].
DODD, IB ;
KALIONIS, B ;
EGAN, JB .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 214 (01) :27-37
[6]   POSITION AND DENSITY EFFECTS ON REPRESSION BY STATIONARY AND MOBILE DNA-BINDING PROTEINS [J].
ELLEDGE, SJ ;
DAVIS, RW .
GENES & DEVELOPMENT, 1989, 3 (02) :185-197
[7]   Transcription through the roadblocks:: the role of RNA polymerase cooperation [J].
Epshtein, V ;
Toulmé, F ;
Rahmouni, AR ;
Borukhov, S ;
Nudler, E .
EMBO JOURNAL, 2003, 22 (18) :4719-4727
[8]   Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position [J].
Eszterhas, SK ;
Bouhassira, EE ;
Martin, DIK ;
Fiering, S .
MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (02) :469-479
[9]   REGULATION OF TRANSCRIPTION FROM TANDEM AND CONVERGENT PROMOTERS [J].
HOROWITZ, H ;
PLATT, T .
NUCLEIC ACIDS RESEARCH, 1982, 10 (18) :5447-5465
[10]   Promoter clearance and escape in prokaryotes [J].
Hsu, LM .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (02) :191-207