Suppression of airway eosinophilia by killed Mycobacterium vaccae-induced allergen-specific regulatory T-cells

被引:418
作者
Zuany-Amorim, C
Sawicka, E
Manlius, C
Le Moine, AL
Brunet, LR
Kemeny, DM
Bowen, G
Rook, G
Walker, C
机构
[1] Novartis Pharmaceut Ltd, Novartis Horsham Res Ctr, Horsham, W Sussex, England
[2] Kings Coll London, Rayne Inst, London WC2R 2LS, England
[3] Free Univ Brussels, Expt Immunol Lab, B-1050 Brussels, Belgium
[4] UCL Royal Free & Univ Coll Med Sch, Windeyer Inst Med Sci, Dept Med Microbiol, London, England
[5] SR Pharma, Ctr Point, London, England
关键词
D O I
10.1038/nm0602-625
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Allergic asthma is a chronic inflammatory disease and despite the introduction of potent and effective drugs, the prevalence has increased substantially over the past few decades(1). The explanation that has attracted the most attention is the 'hygiene hypothesis', which suggests that the increase in allergic diseases is caused by a cleaner environment and fewer childhood infections(2-4). Indeed, certain mycobacterial strains can cause a shift from T-helper cell 2 (Th2) to Th1 immune responses, which may subsequently prevent the development of allergy in mice(5-7). Although the reconstitution of the balance between Th1 and Th2 is an attractive theory, it is unlikely to explain the whole story, as autoimmune diseases characterized by Th1 responses can also benefit from treatment with mycobacteria and their prevalence has also increased in parallel to allergies(8). Here we show that treatment of mice with SRP299, a killed Mycobacterium vaccae-suspension, gives rise to allergen-specific CD4(+)CD45RB(Lo) regulatory T cells, which confer protection against airway inflammation. This specific inhibition was mediated through interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta), as antibodies against IL-10 and TGF-beta completely reversed the inhibitory effect of CD4(+)CD45RB(Lo) T cells. Thus, regulatory T cells generated by mycobacteria treatment may have an essential role in restoring the balance of the immune system to prevent and treat allergic diseases.
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页码:625 / 629
页数:5
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