Identification of feline panleukopenia virus proteins expressed in Purkinje cell nuclei of cats with cerebellar hypoplasia

被引：11

作者：

Poncelet, Luc ^{[1
]}

Heraud, Celine ^{[2
]}

Springinsfeld, Marie ^{[1
]}

Ando, Kunie ^{[2
]}

Kabova, Anna ^{[2
]}

Beineke, Andreas ^{[4
]}

Peeters, Dominique ^{[5
]}

Op De Beeck, Anne ^{[3
]}

Brion, Jean-Pierre ^{[2
]}

机构：

[1] Univ Libre Bruxelles, Fac Med, Lab Anat Biomech & Organogenesis, B-1070 Brussels, Belgium

[2] Univ Libre Bruxelles, Fac Med, Lab Histol Neuroanat & Neuropathol, B-1070 Brussels, Belgium

[3] Univ Libre Bruxelles, Fac Med, B-1070 Brussels, Belgium

[4] Univ Vet Med, Dept Pathol, D-30559 Hannover, Germany

[5] Univ Liege, Fac Vet Med, Dept Compan Anim, B-4000 Liege, Belgium

来源：

VETERINARY JOURNAL | 2013年 / 196卷 / 03期

关键词：

Feline panleukopenia virus; Parvovirus; Capsid proteins; Cerebellar degeneration; Purkinje cells; MINUTE VIRUS; PARVOVIRUS; ACTIVATION; PROMOTER; PHASE; MICE; TAU;

D O I：

10.1016/j.tvjl.2012.10.019

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Parvoviruses depend on initiation of host cell division for their replication. Undefined parvoviral proteins have been detected in Purkinje cells of the cerebellum after experimental feline panleukopenia virus (FPV) infection of neonatal kittens and in naturally occurring cases of feline cerebellar hypoplasia. In this study, a parvoviral protein in the nucleus of Purkinje cells of kittens with cerebellar hypoplasia was shown by immunoprecipitation to be the FPV viral capsid protein VP2. In PCR-confirmed, FPV-associated feline cerebellar hypoplasia, expression of the FPV VP2 protein was demonstrated by immunohistochemistry in Purkinje cell nuclei in 4/10 cases and expression of the FPV non-structural protein NS1 was demonstrated in Purkinje cell nuclei in 5/10 cases. Increased nuclear ERK1 expression was observed in several Purkinje cells in 1/10 kittens. No expression of the G1 and S mitotic phase marker proliferating cell nuclear antigen (PCNA) was evident in Purkinje cell nuclei. These results support the hypothesis that FPV is able to proceed far into its replication cycle in post-mitotic Purkinje cells. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：381 / 387

页数：7

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