Characterization of polymorphic microsatellite markers for the green turtle (Chelonia mydas)

被引：24

作者：

Dutton, Peter H. ^{[1
]}

Frey, Amy ^{[1
]}

机构：

[1] NOAA Fisheries, SW Fisheries Sci Ctr, La Jolla, CA 92037 USA

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 01期

关键词：

Chelonia mydas; green turtle; microsatellites; primers; sea turtles; tetranucleotide repeats; trinucleotide repeats; POPULATION; DNA; PHYLOGEOGRAPHY;

D O I：

10.1111/j.1755-0998.2008.02443.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe primers and polymerase chain reaction conditions to amplify 12 microsatellite loci from the green turtle (Chelonia mydas), including one dinucleotide, four trinucleotide and seven tetranucleotide loci. The primers were tested on 78 individuals from a Pacific population nesting in the Hawaiian Islands. The primer pairs developed in this study yielded an average of 8.33 alleles per locus (range of 3-15 alleles), an average observed heterozygosity of 0.668 (range 0.309-0.910), and an average polymorphic information content of 0.647 (range 0.287-0.894).

引用

页码：354 / 356

页数：3

共 15 条

[1] Population genetics and phylogeography of sea turtles [J].

Bowen, B. W. ;

Karl, S. A. .

MOLECULAR ECOLOGY, 2007, 16 (23) :4886-4907

[2]

Bowen Brian W., 1997, P29

[3] The genetic structure of Australasian green turtles (Chelonia mydas):: exploring the geographical scale of genetic exchange [J].

Dethmers, Kiki E. M. ;

Broderick, Damien ;

Moritz, Craig ;

Fitzsimmons, Nancy N. ;

Limpus, Colin J. ;

Lavery, Shane ;

Whiting, Scott ;

Guinea, Mick ;

Prince, Robert I. T. ;

Kennett, Rod .

MOLECULAR ECOLOGY, 2006, 15 (13) :3931-3946

[4]

Dutton Peter H., 2008, Endangered Species Research, V5, P37, DOI 10.3354/esr00101

[5]

Encalada SE, 1996, MOL ECOL, V5, P473

[6] Philopatry of male marine turtles inferred from mitochondrial DNA markers [J].

FitzSimmons, NN ;

Limpus, CJ ;

Norman, JA ;

Goldizen, AR ;

Miller, JD ;

Moritz, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (16) :8912-8917

[7]

FITZSIMMONS NN, 1995, MOL BIOL EVOL, V12, P432

[8] A SIMPLE AND EFFICIENT NON-ORGANIC PROCEDURE FOR THE ISOLATION OF GENOMIC DNA FROM BLOOD [J].

GRIMBERG, J ;

NAWOSCHIK, S ;

BELLUSCIO, L ;

MCKEE, R ;

TURCK, A ;

EISENBERG, A .

NUCLEIC ACIDS RESEARCH, 1989, 17 (20) :8390-8390

[9] Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis) [J].

Jones, KC ;

Levine, KF ;

Banks, JD .

MOLECULAR ECOLOGY NOTES, 2002, 2 (04) :425-427

[10]

*MAR TURT SPEC GRO, 2004, GREEN TURTL RED LIST

← 1 2 →