Evidence of Schmallenberg virus circulation in ruminants in Greece

被引：22

作者：

Chaintoutis, Serafeim C. ^{[1
]}

Kiossis, Evangelos ^{[2
]}

Giadinis, Nektarios D. ^{[2
]}

Brozos, Christos N. ^{[2
]}

Sailleau, Corinne ^{[3
]}

Viarouge, Cyril ^{[3
]}

Breard, Emmanuel ^{[3
]}

Papanastassopoulou, Maria ^{[1
]}

Zientara, Stephan ^{[3
]}

Papadopoulos, Orestis ^{[1
]}

Dovas, Chrysostomos I. ^{[1
]}

机构：

[1] Aristotle Univ Thessaloniki, Fac Vet Med, Lab Microbiol & Infect Dis, Thessaloniki 54124, Greece

[2] Aristotle Univ Thessaloniki, Fac Vet Med, Clin Farm Anim, Thessaloniki 54627, Greece

[3] French Agcy Food Environm & Occupat Hlth & Safety, UMR Virol, ANSES INRA ENVA, F-94703 Maisons Alfort, France

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2014年 / 46卷 / 01期

关键词：

Schmallenberg virus; Greece; Ruminants; Neutralizing antibodies; Akabane virus; Shamonda virus; SIMBU SEROGROUP; CATTLE; NETHERLANDS; CULICOIDES; INFECTION; EUROPE;

D O I：

10.1007/s11250-013-0449-5

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

During March 2013, we investigated the presence and the levels of Schmallenberg virus (SBV) circulation in three dairy cow herds and three sheep flocks in Central Macedonia, Greece. In two cow herds, a high number of abortions had been observed during the winter. Six bulk-tank milk samples and 147 individual sera were screened for SBV-specific antibodies by ELISA. Positive reactions were obtained from 5 out of 6 bulk-tank milk samples, 58 out of 90 sera from the 3 cow herds, and 2 sera from 2 of the 3 sheep flocks. Twenty-two ELISA-positive sera were tested by serum neutralization test (SNT). SNT confirmed the presence of neutralizing antibodies against SBV in all samples tested, with titers ranging between 1:32 and a parts per thousand yen1:256. No neutralizing antibodies against Akabane virus (AKAV) or Shamonda virus (SHAV) were detected, indicating that neutralizing antibodies against SBV do not cross react with AKAV or SHAV in SNT. ELISA testing of bulk-tank milk samples proved to be convenient and reliable. None of the tested sera was found positive for SBV by real-time RT-PCR, indicating that the sampling was conducted past the viremia stage. This is the first report of SBV circulation in Greece.

引用

页码：251 / 255

页数：5

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