Genetic engineering of microalgae for enhanced biorefinery capabilities

被引:118
作者
Fayyaz, Mehmooda [1 ]
Chew, Kit Wayne [2 ]
Show, Pau Loke [1 ]
Ling, Tau Chuan [3 ]
Ng, I-Son [4 ]
Chang, Jo-Shu [4 ,5 ,6 ]
机构
[1] Univ Nottingham Malaysia, Fac Sci & Engn, Dept Chem & Environm Engn, Jalan Broga, Semenyih 43500, Selangor, Malaysia
[2] Xiamen Univ Malaysia, Sch Energy & Chem Engn, Jalan Sunsuria, Sepang 43900, Selangor, Malaysia
[3] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia
[4] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan
[5] Tunghai Univ, Coll Engn, Dept Chem & Mat Engn, Taichung 407, Taiwan
[6] Tunghai Univ, Res Ctr Smart Sustainable Circular Econ, Taichung 407, Taiwan
关键词
Transgenic algae; Genome editing tools; Risk assessment; Enhanced biomass potential; Algomics; PHYTOENE DESATURASE GENE; CHLAMYDOMONAS-REINHARDTII; LIPID PRODUCTION; DIACYLGLYCEROL ACYLTRANSFERASE; PHAEODACTYLUM-TRICORNUTUM; OLEAGINOUS MICROALGA; SYNTHASE GENE; GENOME; RNA; BIOSYNTHESIS;
D O I
10.1016/j.biotechadv.2020.107554
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microalgae-based bioproducts are in limelight because of their promising future, novel characteristics, the current situation of population needs, and rising prices of rapidly depleting energy resources. Algae-based products are considered as clean sustainable energy and food resources. At present, they are not commercialized due to their high production cost and low yield. In recent years, novel genome editing tools like RNAi, ZNFs, TALENs, and CRISPR/Cas9 are used to enhance the quality and quantity of the desired products. Genetic and metabolic engineering are frequently applied because of their rapid and precise results than random mutagenesis. Omic approaches help enhance biorefinery capabilities and are now in the developing stage for algae. The future is very bright for transgenic algae with increased biomass yield, carbon dioxide uptake rate, accumulating high-value compounds, reduction in cultivation, and production costs, thus reaching the goal in the global algal market and capital flow. However, microalgae are primary producers and any harmful exposure to the wild strains can affect the entire ecosystem. Therefore, strict regulation and monitoring are required to assess the potential risks before introducing genetically modified microalgae into the natural ecosystem.
引用
收藏
页数:13
相关论文
共 146 条
[1]   Genome editing for crop improvement: Challenges and opportunities [J].
Abdallah, Naglaa A. ;
Prakash, Channapatna S. ;
McHughen, Alan G. .
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN, 2015, 6 (04) :183-205
[2]   Biomass and lipid induction strategies in microalgae for biofuel production and other applications [J].
Alishah Aratboni, Hossein ;
Rafiei, Nahid ;
Garcia-Granados, Raul ;
Alemzadeh, Abbas ;
Ruben Morones-Ramirez, Jose .
MICROBIAL CELL FACTORIES, 2019, 18 (01)
[3]   Strategies to unlock lipid production improvement in algae [J].
Anand, V. ;
Kashyap, M. ;
Samadhiya, K. ;
Kiran, B. .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, 2019, 16 (03) :1829-1838
[4]  
[Anonymous], 2014, NAABB FIN REP
[5]   Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis [J].
Baek, Kwangryul ;
Yu, Jihyeon ;
Jeong, Jooyeon ;
Sim, Sang Jun ;
Bae, Sangsu ;
Jin, EonSeon .
BIOTECHNOLOGY AND BIOENGINEERING, 2018, 115 (03) :719-728
[6]   DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins [J].
Baek, Kwangryul ;
Kim, Duk Hyoung ;
Jeong, Jooyeon ;
Sim, Sang Jun ;
Melis, Anastasios ;
Kim, Jin-Soo ;
Jin, EonSeon ;
Bae, Sangsu .
SCIENTIFIC REPORTS, 2016, 6
[7]   PSR1 Is a Global Transcriptional Regulator of Phosphorus Deficiency Responses and Carbon Storage Metabolism in Chlamydomonas reinhardtii [J].
Bajhaiya, Amit K. ;
Dean, Andrew P. ;
Zeef, Leo A. H. ;
Webster, Rachel E. ;
Pittman, Jon K. .
PLANT PHYSIOLOGY, 2016, 170 (03) :1216-1234
[8]   Improvements in algal lipid production: a systems biology and gene editing approach [J].
Banerjee, Avik ;
Banerjee, Chiranjib ;
Negi, Sangeeta ;
Chang, Jo-Shu ;
Shukla, Pratyoosh .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 2018, 38 (03) :369-385
[9]   Microalgal bioengineering for sustainable energy development: Recent transgenesis and metabolic engineering strategies [J].
Banerjee, Chiranjib ;
Singh, Puneet Kumar ;
Shukla, Pratyoosh .
BIOTECHNOLOGY JOURNAL, 2016, 11 (03) :303-314
[10]   Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii [J].
Beckmann, J. ;
Lehr, F. ;
Finazzi, G. ;
Hankamer, B. ;
Posten, C. ;
Wobbe, L. ;
Kruse, O. .
JOURNAL OF BIOTECHNOLOGY, 2009, 142 (01) :70-77