Single-Molecule Detection of 5-Hydroxymethylcytosine in DNA through Chemical Modification and Nanopore Analysis

被引：62

作者：

Li, Wen-Wu ^{[1
,2
]}

Gong, Lingzhi ^{[1
]}

Bayley, Hagan ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

[2] Keele Univ, Inst Sci & Technol Med, Stoke On Trent ST4 7QB, Staffs, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 16期

关键词：

5-hydroxymethylcytosine; DNA methylation; epigenetics; gene sequencing; nanopores; EMBRYONIC STEM-CELLS; SODIUM BISULFITE; BASE-RESOLUTION; MAMMALIAN DNA; 5-METHYLCYTOSINE; GENOME; 5-FORMYLCYTOSINE; METHYLATION; 5-CARBOXYLCYTOSINE; DEAMINATION;

D O I：

10.1002/anie.201300413

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or biotin into DNA. Modified 5hmC bases can be readily distinguished at the single-molecule level using protein nanopore analysis. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4350 / 4355

页数：6

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