Separation of microalgae with different lipid contents by dielectrophoresis

被引:42
作者
Deng, Yu-Luen [1 ]
Chang, Jo-Shu [1 ,2 ,3 ]
Juang, Yi-Je [1 ,2 ,4 ]
机构
[1] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan
[2] Natl Cheng Kung Univ, Res Ctr Energy Technol & Strategy, Tainan 70101, Taiwan
[3] Natl Cheng Kung Univ, Ctr Biosci & Biotechnol, Tainan 70101, Taiwan
[4] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70101, Taiwan
关键词
Microalgae; Lipid content; Dielectrophoresis; Microfluidics; CELL-GROWTH; STRATEGIES;
D O I
10.1016/j.biortech.2012.11.046
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In this study, the effect of the solution conductivity on the behavior of microalgal cells (Chlorella) with different lipid contents under a non-uniform electric field was investigated. It was found that, for the algal cells with 11 wt% lipid content, the crossover frequency is between 2 and 10 MHz when the solution conductivity is within 1.4 and 2.95 mS/cm, and increases as the solution conductivity increases. As to the microalgal cells with 45 wt% lipid content, they experienced negative DEP at frequency below 20 MHz when the solution conductivity is within 2.06 and 2.95 mS/cm. However, positive DEP was observed when the solution conductivity becomes 1.4 mS/cm. In a mixture of the algal cells, those with different lipid contents were successfully separated by DEP at solution conductivity of 2.95 mS/cm and frequency of 20 MHz. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:137 / 141
页数:5
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