Identification and characterization of antigenic epitope of Staphylococcus aureus ClfA adhesin

被引：8
作者：
Li, Yang [1 ]
Liu, Ya-hui [2 ]
Li, Zhi-jie [3 ]
Li, Ya-gang [4 ]
Liu, Ming-yuan [1 ]
Liu, Li-bo [4 ]
Wang, Xiao-guang [4 ]
Wang, Xue-lin [1 ]
Suo, Jian [2 ]
Han, Wen-yu [1 ]
机构：
[1] Jilin Univ, Inst Zoonosis, Minist Educ, Key Lab Zoonosis, Changchun 130062, Peoples R China
[2] Jilin Univ, Hosp 1, Changchun 130021, Jilin Province, Peoples R China
[3] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
[4] Jilin Univ, Hosp 4, Changchun 130062, Peoples R China
来源：
RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 03期
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Staphylococcus aureus; ClfA; Monoclonal antibody; Phage-displayed peptide library; B-cell epitope; CLUMPING FACTOR-A; PHAGE DISPLAY; IN-VITRO; VACCINE; PROTECTION; COMMUNITY; VIRULENCE;
D O I：
10.1016/j.rvsc.2012.10.013
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Staphylococcus aureus ClfA adhesin is a protective antigen that induces partial immunity against S. aureus infection in mice. To identify the antigenic epitope of ClfA, a monoclonal antibody (mAb) D01 against the recombinant protein was produced by the hybridoma technique. The mAb was used to immunoscreen a random phage-displayed peptide library as the immunogen. After three rounds of biopanning, 41 positive clones were identified. Sixteen phage clones were sequenced and their amino acids were deduced. One mimotope (SKVGIDKRRGTA) showed good match with ClfA adhesin at 383-394 aa and the serum of mice induced by the phage clone clearly recognized ClfA adhesin. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：490 / 495
页数：6
相关论文
共 24 条
[1]   How microorganisms avoid phagocyte attraction [J].
Bestebroer, Jovanka ;
de Haas, Carla J. C. ;
van Strijp, Jos A. G. .
FEMS MICROBIOLOGY REVIEWS, 2010, 34 (03) :395-414
[2]   In vivo and in vitro demonstration that Staphylococcus aureus is an intracellular pathogen in the presence or absence of fibronectin-binding proteins [J].
Brouillette, E ;
Grondin, G ;
Shkreta, L ;
Lacasse, P ;
Talbot, BG .
MICROBIAL PATHOGENESIS, 2003, 35 (04) :159-168
[3]   DNA immunization against the clumping factor A (ClfA) of Staphylococcus aureus [J].
Brouillette, E ;
Lacasse, P ;
Shkreta, L ;
Bélanger, J ;
Grondin, G ;
Diarra, MS ;
Fournier, S ;
Talbot, BG .
VACCINE, 2002, 20 (17-18) :2348-2357
[4]   Community-Associated Methicillin-Resistant Staphylococcus aureus: Epidemiology and Clinical Consequences of an Emerging Epidemic [J].
David, Michael Z. ;
Daum, Robert S. .
CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (03) :616-+
[5]   Community-associated meticillin-resistant Staphylococcus aureus [J].
DeLeo, Frank R. ;
Otto, Michael ;
Kreiswirth, Barry N. ;
Chambers, Henry F. .
LANCET, 2010, 375 (9725) :1557-1568
[6]   Evaluation of Clumping Factor A Binding Region A in a Subunit Vaccine against Staphylococcus aureus-Induced Mastitis in Mice [J].
Gong, Rui ;
Hu, Changmin ;
Xu, Haiyang ;
Guo, Aizhen ;
Chen, Huanchun ;
Zhang, Guangzhi ;
Shi, Lei .
CLINICAL AND VACCINE IMMUNOLOGY, 2010, 17 (11) :1746-1752
[7]   Staphylococcus aureus nasal carriage rate and associated risk factors in individuals in the community [J].
Halablab, M. A. ;
Hijazi, S. M. ;
Fawzi, M. A. ;
Araj, G. F. .
EPIDEMIOLOGY AND INFECTION, 2010, 138 (05) :702-706
[8]   Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant [J].
Josefsson, E ;
Hartford, O ;
O'Brien, L ;
Patti, JM ;
Foster, T .
JOURNAL OF INFECTIOUS DISEASES, 2001, 184 (12) :1572-1580
[9]   Fibrinogen Binding Sites P336 and Y338 of Clumping Factor A Are Crucial for Staphylococcus aureus Virulence [J].
Josefsson, Elisabet ;
Higgins, Judy ;
Foster, Timothy J. ;
Tarkowski, Andrej .
PLOS ONE, 2008, 3 (05)
[10]   Comparative genomics of epidemic versus sporadic Staphylococcus aureus strains does not reveal molecular markers for epidemicity [J].
Kuhn, Gerrit ;
Koessler, Thibaud ;
Melles, Damian C. ;
Francois, Patrice ;
Huyghe, Antoine ;
Dunman, Paul ;
Vos, Margreet C. ;
Zanetti, Giorgio ;
Schrenzel, Jacques ;
van Belkum, Alex ;
Blanc, Dominique S. .
INFECTION GENETICS AND EVOLUTION, 2010, 10 (01) :89-96
123