Expression and enzymatic activity of glutathione s-transferase in tissues of Boophilus microplus females

被引：11

作者：

De Freitas, Daniela R. J. ^{[1
]}

Vaz Junior, Itabajara Da S. ^{[1
,2
]}

Masuda, Aoi ^{[1
,3
]}

机构：

[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Fac Vet, BR-91501970 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Dept Biol Mol & Biotecnol, BR-91501970 Porto Alegre, RS, Brazil

来源：

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA | 2008年 / 17卷 / 02期

关键词：

Rhipicephalus microplus; antioxidant; senescence; GST; preovipostion;

D O I：

10.1590/S1984-29612008000200007

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cellular detoxification and excretion enzymes are important to the maintenance of cellular homeostasis. In this work mRNA transcription, protein expression and enzymatic activity of Glutathione S-transferases (GSTs), enzymes involved in the excretion of endo and xenobiotic compounds were analyzed. These parameters are elements believed to protect cells against chemical toxicity and oxidative stress in different tissues (salivary gland, ovary and synganglion) from partially engorged females and engorged females of Boophilus microplus. The results presented showed elevated GST activity in partially engorged females. The enzymatic activity decreased during the preoviposition period in engorged females. GST mRNA transcription was detected in salivary glands and synganglion from partially engorged and engorged females, but not in ovary. The results of this work help to elucidate the role of GST in tick development and assist in the understanding of the importance of GST in tick females during the preparation for oviposition.

引用

页码：99 / 104

页数：6

共 40 条

[1] Purification and catalytic properties of glutathione transferase from the Hepatopancreas of crayfish Macrobrachium vollenhovenii (Herklots) [J].