MicroRNA-148a-3p inhibits cancer progression and is a novel screening biomarker for gastric cancer

被引：20

作者：

Bao, Chenhui ^{[1
]}

Guo, Lin ^{[1
]}

机构：

[1] China Med Univ, Dept Gastrointestinal Surg, ShengJing Hosp, 36 Sanhao St, Shenyang 110004, Liaoning, Peoples R China

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2020年 / 34卷 / 10期

关键词：

biomarker; gastric cancer; microRNA; miR-148a-3p; COMPETITIVE ENDOGENOUS RNA; MIR-148A-3P SUPPRESSES; PROLIFERATION; STATISTICS; INVASION;

D O I：

10.1002/jcla.23454

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Purpose Dysregulation of miR-148a-3p in gastric cancer was reported. However, the diagnostic potential and biological function of miR-148a-3p in gastric cancer progression is not fully studied. Methods Bioinformatics analysis and RT-qPCR assay were performed to analyze the expression of miR-148a-3p in gastric cancer tissues and plasma of gastric cancer patients. Receiver operating characteristic curve analysis was performed to analyze the diagnostic value of miR-148a-3p. In vitro proliferation, apoptosis, migration, invasion, sphere formation assay and Western blotting assay were performed to evaluate the biological function of miR-148a-3p in gastric cancer progression. Results miR-148a-3p was significantly down-regulated in both gastric cancer patients' tissue and plasma samples. Plasma miR-148a-3p showed promising efficacy for gastric cancer diagnosis. Overexpression of miR-148a-3p could inhibit the proliferative phenotype, metastatic phenotype, and cancer stem-like properties of gastric cancer cells. Conclusions miR-148a-3p inhibits cancer progression and is a novel diagnostic biomarker for gastric cancer.

引用

页数：9

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