Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling

被引：23

作者：

Huang, Yi-Ping ^{[1
]}

Ni, Chien-Hang ^{[2
]}

Lu, Chi-Cheng ^{[3
]}

Chiang, Jo-Hua ^{[3
]}

Yang, Jai-Sing ^{[4
]}

Ko, Yang-Ching ^{[5
]}

Lin, Jing-Pin ^{[6
]}

Kuo, Jehn-Hwa ^{[7
,8
]}

Chang, Shu-Jen ^{[9
]}

Chung, Jing-Gung ^{[10
,11
,12
]}

机构：

[1] China Med Univ, Dept Physiol, Taichung 404, Taiwan

[2] I Shou Univ, E DA Hosp, Dept Chinese Med, Kaohsiung 824, Taiwan

[3] Natl Chung Hsing Univ, Dept Life Sci, Taichung 402, Taiwan

[4] China Med Univ, Dept Pharmacol, Taichung 404, Taiwan

[5] St Martin De Porres Hosp, Dept Internal Med, Div Pulm & Crit Care Med, Chiayi 600, Taiwan

[6] China Med Univ, Sch Chinese Med, Taichung 404, Taiwan

[7] Cent Taiwan Univ Sci & Technol, Taichung 406, Taiwan

[8] Jen Ai Hosp, Dept Urol, Taichung 412, Taiwan

[9] China Med Univ, Sch Pharm, Taichung 404, Taiwan

[10] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan

[11] China Med Univ, Dept Microbiol, Taichung 404, Taiwan

[12] Asia Univ, Dept Biotechnol, Taichung 413, Taiwan

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

关键词：

CANCER CELLS; PROTEIN PHOSPHATASE; GROWTH-INHIBITION; DOWN-REGULATION; CYCLE ARREST; ORAL-CANCER; APOPTOSIS; NORCANTHARIDIN; METASTASIS; EXPRESSION;

D O I：

10.1155/2013/190281

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Cancer metastasis becomes an initial cause of cancer death in human population. In many cancers, it has been shown that the high levels of matrix metalloproteinase (MMP)-2 and/or MMP-9 are associated with the invasive phenotypes of cancer cells. In this study, we investigated the effects of cantharidin, a derivative of blister beetles which is one of the traditional Chinese medicines, on the adhesion, migration, and invasion of human bladder cancer TSGH-8301 cells. Cantharidin effectively suppressed TSGH-8301 cell adhesion, migration, and invasion in a concentration-dependent manner. Results from Western blotting, RT-PCR, and gelatin zymography assays indicated that cantharidin blocked the protein levels, gene expression (mRNA), and activities of MMP-2 and -9 in TSGH-8301 cells. Cantharidin also significantly suppressed the protein expressions of p-p38 and p-JNK1/2 in TSGH-8301 cells. Taken together, cantharidin was suggested to present antimetastatic potential via suppressing the levels of MMP-2 and MMP-9 expression that might be mediated by targeting the p38 and JNK1/2 MAPKs pathway in TSGH-8301 human bladder cancer cells.

引用

页数：8

共 42 条

[1] Proteomic approaches to study epigallocatechin gallate-provoked apoptosis of TSGH-8301 human urinary bladder carcinoma cells: Roles of AKT and heat shock protein 27-modulated intrinsic apoptotic pathways [J].