SSAP Markers Based on a Novel Ty1-copia Like Element Are a Powerful Tool for the Assessment of Genetic Diversity in Rye (Secale cereale L.) Inbred Lines

被引：4

作者：

Bolibok-Bragoszewska, H. ^{[1
]}

Zabierzewska, N. ^{[1
]}

Hromada-Judycka, A. ^{[1
]}

Krzewska, L. ^{[1
]}

机构：

[1] Warsaw Univ Life Sci SGGW, Dept Plant Genet Breeding & Biotechnol, Fac Hort & Landscape Architecture, PL-02776 Warsaw, Poland

来源：

CEREAL RESEARCH COMMUNICATIONS | 2012年 / 40卷 / 02期

关键词：

genetic diversity; molecular markers; retrotransposon; rye; SSAP; Secale cereale; Ty1-copia; RETROTRANSPOSON-BASED SSAP; MOLECULAR MARKER; LTR SEQUENCES; GENOME; LINKAGE; AFLP; ARM;

D O I：

10.1556/CRC.40.2012.2.4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A partial sequence of a Ty1-copia retrotransposon was isolated from the genome of the rye (Secale cereale L.) inbred line L318 using degenerate primers targeted at the RNaseH sequence. Its putative 3'LTR region exhibited similarity to LTRs of known plant retrotransposons such as BARE-1, while no significant similarity to known rye retrotransposon sequences was found. A Sequence Specific Amplification Polymorphism (SSAP) protocol employing primer designed to correspond to the putative polypurine tract was developed and used for analysis of 30 rye inbred lines. Values of the parameters calculated to evaluate the efficiency of the method as well as the results of cluster analysis demonstrate that it is a powerful tool for genetic diversity analyses of rye.

引用

页码：204 / 209

页数：6

共 25 条

[1] SSAP Markers Based on a Novel Ty1-Copia Like Element Are a Powerful Tool for the Assessment of Genetic Diversity in Rye (Secale Cereale L.) Inbred Lines
H. Bolibok-Brbągoszewska
N. Zabierzewska
A. Hromada-Judycka
L. Krzewska
Cereal Research Communications, 2012, 40 : 204 - 209
[2] Identification of Bilby, a diverged centromeric Ty1-copia retrotransposon family from cereal rye (Secale cereale L.)
Francki, MG
GENOME, 2001, 44 (02) : 266 - 274
[3] Development of Ty1-copia retrotransposon-based SSAP molecular markers for the study of genetic diversity in peach
Jiao, Yun
Ma, Rui-juan
Shen, Zhi-jun
Yu, Ming-liang
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2014, 57 : 270 - 277
[4] Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers
Shang, Hai-Ying
Wei, Yu-Ming
Wang, Xiao-Rong
Zheng, You-Liang
GENETICS AND MOLECULAR BIOLOGY, 2006, 29 (04) : 685 - 691
[5] Ty1-copia retrotransposon-based SSAP marker development in cashew (Anacardium occidentale L.)
N. H. Syed
S. Sureshsundar
M. J. Wilkinson
B. S. Bhau
J. J. V. Cavalcanti
A. J. Flavell
Theoretical and Applied Genetics, 2005, 110 : 1195 - 1202
[6] Ty1-copia retrotransposon-based SSAP marker development in cashew (Anacardium occidentale L.)
Syed, NH
Sureshsundar, S
Wilkinson, MJ
Bhau, BS
Cavalcanti, JJV
Flavell, AJ
THEORETICAL AND APPLIED GENETICS, 2005, 110 (07) : 1195 - 1202
[7] Efficiency of different PCR-based marker systems in assessing genetic diversity among winter rye (Secale cereale L.) inbred lines
Hanna Bolibok
Monika Rakoczy-Trojanowska
Aneta Hromada
Robert Pietrzykowski
Euphytica, 2005, 146 : 109 - 116
[8] Efficiency of different PCR-based marker systems in assessing genetic diversity among winter rye (Secale cereale L.) inbred lines
Bolibok, H
Rakoczy-Trojanowska, M
Hromada, A
Pietrzykowski, R
EUPHYTICA, 2005, 146 (1-2) : 109 - 116
[9] GENETIC DIVERSITY OF CZECHOSLOVAK ORIGIN RYE VARIETIES (SECALE CEREALE L.) BASED ON PROTEIN POLYMORPHISM
Petrovicova, Lenka
Galova, Zdenka
Chnapek, Milan
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES, 2014, 3 : 267 - 269
[10] Evaluation of rye (Secale cereale L.) inbred lines and their crosses for tissue culture response and stable genetic transformation of homozygous rye inbred line L22 by biolistic gene transfer
Popelka, JC
Altpeter, F
THEORETICAL AND APPLIED GENETICS, 2003, 107 (04) : 583 - 590

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