Preliminary study on puffer fish proteome - Species identification of puffer fish by two-dimensional electrophoresis

被引:18
作者
Chen, TY
Shiau, CY
Wei, CI
Hwang, DF [1 ]
机构
[1] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung 202, Taiwan
[2] Oklahoma State Univ, Coll Human Environm Sci, Stillwater, OK 74078 USA
关键词
two-dimensional electrophoresis; puffer fish; species identification; proteome; protein;
D O I
10.1021/jf035033n
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins resolved into 171-260 spots in the 2DE gels, with a pl range of 3-10 and molecular mass range of 7.4-205.0 kDa, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pl values of 3.5-7.0, and molecular masses of 7.4-45.0 kDa were well-resolved and were good for species comparison. The more acidic proteins of lower molecular masses showed species specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2DE protein patterns.
引用
收藏
页码:2236 / 2241
页数:6
相关论文
共 53 条
[11]  
2-J
[12]   Identification of fish species after cooking by SDS-PAGE and urea IEF:: A collaborative study [J].
Etienne, M ;
Jérôme, M ;
Fleurence, J ;
Rehbein, H ;
Kündiger, R ;
Mendes, R ;
Costa, H ;
Pérez-Martín, R ;
Piñeiro-González, C ;
Craig, A ;
Mackie, I ;
Yman, IM ;
Ferm, M ;
Martínez, I ;
Jessen, F ;
Smelt, A ;
Luten, J .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (07) :2653-2658
[13]   USE OF CAPILLARY ZONE ELECTROPHORESIS FOR FISH SPECIES IDENTIFICATION - DIFFERENTIATION OF FLATFISH SPECIES [J].
GALLARDO, JM ;
SOTELO, CG ;
PINEIRO, C ;
PEREZMARTIN, RI .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (05) :1238-1244
[14]  
Gazzaz Sahl S., 1993, Reviews in Fisheries Science, V1, P1
[15]  
Görg A, 2000, ELECTROPHORESIS, V21, P1037, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO
[16]  
2-V
[17]  
Hanash SM, 2000, ELECTROPHORESIS, V21, P1202, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1202::AID-ELPS1202>3.3.CO
[18]  
2-9
[19]   Development of a DNA-based method aimed at identifying the fish species present in food products [J].
Hold, GL ;
Russell, VJ ;
Pryde, SE ;
Rehbein, H ;
Quinteiro, J ;
Vidal, R ;
Rey-Mendez, M ;
Sotelo, CG ;
Pérez-Martin, RI ;
Santos, AT ;
Rosa, C .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (03) :1175-1179
[20]   Identification of tetrodotoxin and fish species in an adulterated dried mullet roe implicated in food poisoning [J].
Hsieh, YW ;
Hwang, PA ;
Pan, HH ;
Chen, JB ;
Hwang, DF .
JOURNAL OF FOOD SCIENCE, 2003, 68 (01) :142-146