Preliminary study on puffer fish proteome - Species identification of puffer fish by two-dimensional electrophoresis

被引：18

作者：

Chen, TY

Shiau, CY

Wei, CI

Hwang, DF ^{[1
]}

机构：

[1] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung 202, Taiwan

[2] Oklahoma State Univ, Coll Human Environm Sci, Stillwater, OK 74078 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 08期

关键词：

two-dimensional electrophoresis; puffer fish; species identification; proteome; protein;

D O I：

10.1021/jf035033n

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins resolved into 171-260 spots in the 2DE gels, with a pl range of 3-10 and molecular mass range of 7.4-205.0 kDa, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pl values of 3.5-7.0, and molecular masses of 7.4-45.0 kDa were well-resolved and were good for species comparison. The more acidic proteins of lower molecular masses showed species specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2DE protein patterns.

引用

页码：2236 / 2241

页数：6

共 53 条

[1] Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes [J].

Aparicio, S ;

Chapman, J ;

Stupka, E ;

Putnam, N ;

Chia, J ;

Dehal, P ;

Christoffels, A ;

Rash, S ;

Hoon, S ;

Smit, A ;

Gelpke, MDS ;

Roach, J ;

Oh, T ;

Ho, IY ;

Wong, M ;

Detter, C ;

Verhoef, F ;

Predki, P ;

Tay, A ;

Lucas, S ;

Richardson, P ;

Smith, SF ;

Clark, MS ;

Edwards, YJK ;

Doggett, N ;

Zharkikh, A ;

Tavtigian, SV ;

Pruss, D ;

Barnstead, M ;

Evans, C ;

Baden, H ;

Powell, J ;

Glusman, G ;

Rowen, L ;

Hood, L ;

Tan, YH ;

Elgar, G ;

Hawkins, T ;

Venkatesh, B ;

Rokhsar, D ;

Brenner, S .

SCIENCE, 2002, 297 (5585) :1301-1310

[2] THE USE OF HPLC PROTEIN PROFILES IN FISH SPECIES IDENTIFICATION [J].

ARMSTRONG, SG ;

LEACH, DN ;

WYLLIE, SG .

FOOD CHEMISTRY, 1992, 44 (02) :147-155

[3] CHARACTERIZATION OF THE PUFFERFISH (FUGU) GENOME AS A COMPACT MODEL VERTEBRATE GENOME [J].

BRENNER, S ;

ELGAR, G ;

SANDFORD, R ;

MACRAE, A ;

VENKATESH, B ;

APARICIO, S .

NATURE, 1993, 366 (6452) :265-268

[4] Identification of species and measurement of tetrodotoxin in dried dressed fillets of the puffer fish, Lagocephalus lunaris [J].

Chen, TY ;

Hsieh, YW ;

Tsai, YH ;

Shiau, CY ;

Hwang, DF .

JOURNAL OF FOOD PROTECTION, 2002, 65 (10) :1670-1673

[5] Electrophoretic identification of muscle proteins in 7 puffer species [J].

Chen, TY ;

Hwang, DF .

JOURNAL OF FOOD SCIENCE, 2002, 67 (03) :936-942

[6]

CHEN TY, 2003, IN PRESS FOOD CHEM

[7]

Cheng CA, 2001, J FOOD DRUG ANAL, V9, P232

[8] Small is beautiful: Comparative genomics with the pufferfish (Fugu rubripes) [J].

Elgar, G ;

Sandford, R ;

Aparicio, S ;

Macrae, A ;

Venkatesh, B ;

Brenner, S .

TRENDS IN GENETICS, 1996, 12 (04) :145-150

[9] Species identification of formed fishery products and high pressure-treated fish by electrophoresis:: a collaborative study [J].

Etienne, M ;

Jérôme, M ;

Fleurence, J ;

Rehbein, H ;

Kündiger, R ;

Mendes, R ;

Costa, H ;

Martinez, I .

FOOD CHEMISTRY, 2001, 72 (01) :105-112

[10]

Etienne M, 1999, ELECTROPHORESIS, V20, P1923, DOI 10.1002/(SICI)1522-2683(19990701)20:10<1923::AID-ELPS1923>3.0.CO

← 1 2 3 4 5 6 →