Maturity and age influence chief cell ability to transdifferentiate into metaplasia

被引:29
作者
Weis, Victoria G. [2 ,5 ]
Petersen, Christine P. [1 ,4 ,5 ]
Weis, Jared A. [3 ]
Meyer, Anne R. [4 ,5 ]
Choi, Eunyoung [1 ,2 ,5 ]
Mills, Jason C. [6 ,7 ]
Goldenring, James R. [1 ,2 ,4 ,5 ]
机构
[1] Nashville Vet Affairs Med Ctr, Nashville, TN USA
[2] Vanderbilt Univ, Med Ctr, Sect Surg Sci, Nashville, TN USA
[3] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA
[4] Vanderbilt Univ, Dept Cell & Dev Biol, 221 Kirkland Hall, Nashville, TN 37235 USA
[5] Vanderbilt Univ, Med Ctr, Epithelial Biol Ctr, 10435-G MRB 4,2213 Garland Ave, Nashville, TN 37232 USA
[6] Washington Univ, Dept Med Pathol & Immunol, St Louis, MO USA
[7] Washington Univ, Dept Dev Biol, St Louis, MO USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2017年 / 312卷 / 01期
关键词
SPEM; spasmolytic polypeptide-expressing metaplasia; lineage mapping; Mist1; transdifferentiation; progenitor cell; POLYPEPTIDE-EXPRESSING METAPLASIA; TRANSCRIPTION FACTOR MIST1; ACUTE OXYNTIC ATROPHY; MOUSE STOMACH; EPITHELIAL-CELLS; GASTRIC CARCINOGENESIS; ZYMOGENIC CELLS; PARIETAL-CELLS; DEFICIENT MICE; DYNAMICS;
D O I
10.1152/ajpgi.00326.2016
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The plasticity of gastric chief cells is exemplified by their ability to transdifferentiate into spasmolytic polypeptide-expressing metaplasia (SPEM) after parietal cell loss. We sought to determine if chief cell maturity is a limiting factor in the capacity to transdifferentiate. Mist1(-/-) mice, previously shown to form only immature chief cells, were treated with DMP-777 or L635 to study the capability of these immature chief cells to transdifferentiate into a proliferative metaplastic lineage after acute parietal cell loss. Mist1(-/-) mice treated with DMP-777 showed fewer chief cell to SPEM transitions. Mist1(-/-) mice treated with L635 demonstrated significantly fewer proliferative SPEM cells compared with control mice. Thus immature chief cells were unable to transdifferentiate efficiently into SPEM after acute parietal cell loss. To determine whether chief cell age affects transdifferentiation into SPEM, we used tamoxifen to induce YFP expression in chief cells of Mist1(CreER/+); Rosa(YFP) mice and subsequently treated the cells with L635 to induce SPEM at 1 to 3.5 mo after tamoxifen treatment. After L635 treatment to induce acute parietal cell loss, 43% of all YFP-positive cells at 1 mo posttamoxifen were SPEM cells, of which 44% of these YFP-positive SPEM cells were proliferative. By 2 mo after tamoxifen induction, only 24% of marked SPEM cells were proliferating. However, by 3.5 mo after tamoxifen induction, only 12% of marked chief cells transdifferentiated into SPEM and none were proliferative. Thus, as chief cells age, they lose their ability to transdifferentiate into SPEM and proliferate. Therefore, both functional maturation and age limit chief cell plasticity. NEW & NOTEWORTHY Previous investigations have indicated that spasmolytic polypeptide-expressing metaplasia (SPEM) in the stomach arises from transdifferentiation of chief cells. Nevertheless, the intrinsic properties of chief cells that influence transdifferentiation have been largely unknown. We now report that the ability to transdifferentiate into SPEM is impaired in chief cells that lack full functional maturation, and as chief cells age, they lose their ability to transdifferentiate. Thus chief cell plasticity is dependent on both cell age and maturation.
引用
收藏
页码:G67 / G76
页数:10
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