Determination of allatostatin levels in relation to the gonadotropic cycle in the female of Blattella germanica (L.) (Dictyoptera, Blattellidae)

被引:12
作者
Vilaplana, L [1 ]
Maestro, JL [1 ]
Piulachs, MD [1 ]
Bellés, X [1 ]
机构
[1] CSIC, Cid, Dept Physiol & Mol Biodivers, Inst Mol Biol, ES-08034 Barcelona, Spain
关键词
allatostatin; Blattella germanica; brain; ELISA; haemolymph; midgut;
D O I
10.1046/j.1365-3032.1999.00132.x
中图分类号
Q96 [昆虫学];
学科分类号
摘要
A polyclonal antibody against the allatostatin BLAST-3 (AGSDGRLYSFGL-NH2) of the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae) has been raised and characterized, and an ELISA (enzyme-linked immunosorbent assay) for allatostatin quantification has been developed. Allatostatin contents in brain, midgut and haemolymph have been measured in females of B. germanica during the first gonadotropic cycle. Brain allatostatin content increases steadily from adult emergence to the formation of the first ootheca. The values range from 2 ng/brain on the day of adult emergence to 25 ng/brain when the insect forms the ootheca 8 days later. In the midgut, the pattern is similar but the values are about half those of the brain. Allatostatin concentrations in the haemolymph after HPLC separation are in the nanomolar range. The occurrence of allatostatins in the haemolymph suggests that these peptides can act through a humoral pathway, as well as via nerves. The allatostatin content of both brain and midgut are high while the female is transporting the ootheca, which suggests that these peptides could be related to the low metabolic status characterising the period of oothecal transport.
引用
收藏
页码:213 / 219
页数:7
相关论文
共 42 条
[1]   INVITRO BIOSYNTHESIS OF JH-III BY THE CORPORA ALLATA OF ADULT FEMALES OF BLATTELLA-GERMANICA (L) [J].
BELLES, X ;
CASAS, J ;
MESSEGUER, A ;
PIULACHS, MD .
INSECT BIOCHEMISTRY, 1987, 17 (07) :1007-1010
[2]   The molecular evolution of the allatostatin precursor in cockroaches [J].
Bellés, X ;
Graham, LA ;
Bendena, WG ;
Ding, Q ;
Edwards, JP ;
Weaver, RJ ;
Tobe, SS .
PEPTIDES, 1999, 20 (01) :11-22
[3]   ALLATOSTATIC NEUROPEPTIDES FROM THE COCKROACH BLATTELLA-GERMANICA (L) (DICTYOPTERA, BLATTELLIDAE) - IDENTIFICATION, IMMUNOLOCALIZATION AND ACTIVITY [J].
BELLES, X ;
MAESTRO, JL ;
PIULACHS, MD ;
JOHNSEN, AH ;
DUVE, H ;
THORPE, A .
REGULATORY PEPTIDES, 1994, 53 (03) :237-247
[4]   Allatostatins: Diversity in structure and function of an insect neuropeptide family [J].
Bendena, WG ;
Garside, CS ;
Yu, CG ;
Tobe, SS .
NEUROPEPTIDES IN DEVELOPMENT AND AGING, 1997, 814 :53-66
[5]  
Davis NT, 1997, J COMP NEUROL, V385, P265, DOI 10.1002/(SICI)1096-9861(19970825)385:2<265::AID-CNE6>3.0.CO
[6]  
2-#
[7]   Comparison of the allatostatin neuropeptide precursors in the distantly related cockroaches Periplaneta americana and Diploptera punctata [J].
Ding, Q ;
Donly, BC ;
Tobe, SS ;
Bendena, WG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 234 (03) :737-746
[8]   MOLECULAR-CLONING OF THE GENE FOR THE ALLATOSTATIN FAMILY OF NEUROPEPTIDES FROM THE COCKROACH DIPLOPTERA-PUNCTATA [J].
DONLY, BC ;
DING, Q ;
TOBE, SS ;
BENDENA, WG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :8807-8811
[9]   Identification of the dipteran Leu-callatostatin peptide family: The pattern of precursor processing revealed by isolation studies in Calliphora vomitoria [J].
Duve, H ;
Johnsen, AH ;
Maestro, JL ;
Scott, AG ;
East, PD ;
Thorpe, A .
REGULATORY PEPTIDES, 1996, 67 (01) :11-19
[10]   Lepidopteran peptides of the allatostatin superfamily [J].
Duve, H ;
Johnsen, AH ;
Maestro, JL ;
Scott, AG ;
Winstanley, D ;
Davey, M ;
East, PD ;
Thorpe, A .
PEPTIDES, 1997, 18 (09) :1301-1309