Identification of mouse palmitoyl-coenzyme A Δ9-desaturase

被引:85
|
作者
Miyazaki, M [1 ]
Bruggink, SM
Ntambi, JM
机构
[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Nutr Sci, Madison, WI 53706 USA
关键词
stearoyl-CoA desaturase; oleate; palmitoleate; substrate specificity;
D O I
10.1194/jlr.C500025-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stearoyl-coenzyme A desaturase (SCD) catalyzes the desaturation of saturated fatty acids to monounsaturated fatty acids in mammalian cells. Currently, there are four known enzymatic isoforms (SCD1-SCD4) in the mouse genome. The physiological roles for multiple SCD isoforms and their substrate specificities are unknown at present. We report here distinct substrate specificities for the mouse SCD isoforms. Each SCD isoform was able to complement the ole1 mutation in Saccharomyces cerevisiae through heterologous expression of transgenic SCD. Fatty acid analysis showed that mouse SCD1, SCD2, and SCD4 desaturate both C18:0 and C16:0, whereas mouse SCD3 uses C16: 0 but not C18:0. We identify SCD3 as a mammalian palmitotyl-CoA Delta 9-desaturase, and its existence in mouse helps explain distinct physiological roles for each SCD isoform. - Miyazaki, M., S. M. Bruggink, and J. M. Ntambi. Identification of mouse palmitoyl-coenzyme A Delta 9-desaturase.
引用
收藏
页码:700 / 704
页数:5
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