Structure and functional expression of the cloned mouse neuronal high-affinity glutamate transporter

被引：10
|
作者：
MaenoHikichi, Y
Tanaka, K
Shibata, T
Watanabe, M
Inoue, Y
Mukainaka, Y
Wada, K
机构：
[1] NATL CTR NEUROL & PSYCHIAT,NATL INST NEUROSCI,DEPT DEGENERAT NEUROL DIS,KODAIRA,TOKYO 187,JAPAN
[2] HOKKAIDO UNIV,SCH MED,DEPT ANAT,SAPPORO,HOKKAIDO 060,JAPAN
来源：
MOLECULAR BRAIN RESEARCH | 1997年 / 48卷 / 01期
基金：
日本科学技术振兴机构;
关键词：
glutamate/aspartate transporter; cerebellum; mouse; cDNA; in situ hybridization;
D O I：
10.1016/S0169-328X(97)00169-1
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
A cDNA encoding a neuron-specific glutamate transporter was isolated from mouse cerebellum and characterized, Expression of this cDNA in HEK293 cells induced high-affinity uptake of L-glutamate that was dependent on external Na+, The deduced amino-acid sequence of the cDNA shares 96% identity with its human homologue. Northern blot analysis of mouse tissues revealed that the mRNA of this transporter is specific to the brain, In situ hybridization in mouse brain sections showed that the mRNA is expressed densely and selectively in cell. bodies of Purkinje cells. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：176 / 180
页数：5
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