Quantifying protein dynamics and stability in a living organism

被引：40
|
作者：
Feng, Ruopei [1 ]
Gruebele, Martin [1 ,2 ,3 ]
Davis, Caitlin M. [1 ,2 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
[3] Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA
来源：
NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期
基金：
美国国家科学基金会;
关键词：
EXCLUDED-VOLUME; TRANSGENESIS; CRYSTALLIN; GENE;
D O I：
10.1038/s41467-019-09088-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperaturejump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.
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页数：7
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