Quantifying protein dynamics and stability in a living organism

被引：40

作者：

Feng, Ruopei ^{[1
]}

Gruebele, Martin ^{[1
,2
,3
]}

Davis, Caitlin M. ^{[1
,2
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[3] Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

美国国家科学基金会;

关键词：

EXCLUDED-VOLUME; TRANSGENESIS; CRYSTALLIN; GENE;

D O I：

10.1038/s41467-019-09088-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperaturejump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.

引用

页数：7

共 50 条

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