Quantifying protein dynamics and stability in a living organism

被引：40
|
作者：
Feng, Ruopei [1 ]
Gruebele, Martin [1 ,2 ,3 ]
Davis, Caitlin M. [1 ,2 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
[3] Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA
来源：
NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期
基金：
美国国家科学基金会;
关键词：
EXCLUDED-VOLUME; TRANSGENESIS; CRYSTALLIN; GENE;
D O I：
10.1038/s41467-019-09088-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperaturejump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.
引用
收藏
页数：7
相关论文
共 50 条
  • [1] Quantifying protein dynamics and stability in a living organism
    Ruopei Feng
    Martin Gruebele
    Caitlin M. Davis
    Nature Communications, 10
  • [2] Protein folding stability and dynamics imaged in a living cell
    Ebbinghaus, Simon
    Dhar, Apratim
    McDonald, Douglas
    Gruebele, Martin
    NATURE METHODS, 2010, 7 (04) : 319 - 323
  • [3] Protein folding stability and dynamics imaged in a living cell
    Ebbinghaus S.
    Dhar A.
    McDonald J.D.
    Gruebele M.
    Nature Methods, 2010, 7 (4) : 319 - 323
  • [4] Quantifying a protein-protein interaction in living cells
    Speer, Shannon
    Guseman, Alex
    Pielak, Gary
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 258
  • [5] Quantifying a Protein-Protein Interaction in Living Cells
    Speer, Shannon L.
    Guseman, Alex J.
    Pielak, Gary J.
    BIOPHYSICAL JOURNAL, 2019, 116 (03) : 61A - 61A
  • [6] Deciphering dynamics of clathrin-mediated endocytosis in a living organism
    Ferguson, Joshua P.
    Willy, Nathan M.
    Heidotting, Spencer P.
    Huber, Scott D.
    Webber, Matthew J.
    Kural, Comert
    JOURNAL OF CELL BIOLOGY, 2016, 214 (03): : 347 - 358
  • [7] Protein Stability in Living Cells
    Monteith, William B.
    Pielak, Gary J.
    BIOPHYSICAL JOURNAL, 2014, 106 (02) : 736A - 736A
  • [8] Optimal fluorescent protein tags for quantifying protein oligomerization in living cells
    Valentin Dunsing
    Madlen Luckner
    Boris Zühlke
    Roberto A. Petazzi
    Andreas Herrmann
    Salvatore Chiantia
    Scientific Reports, 8
  • [9] Optimal fluorescent protein tags for quantifying protein oligomerization in living cells
    Dunsing, Valentin
    Luckner, Madlen
    Zuehlke, Boris
    Petazzi, Roberto A.
    Herrmann, Andreas
    Chiantia, Salvatore
    SCIENTIFIC REPORTS, 2018, 8
  • [10] Dynamics of macrophage polarization support Salmonella persistence in a whole living organism
    Leiba, Jade
    Sipka, Tamara
    Begon-Pescia, Christina
    Bernardello, Matteo
    Tairi, Sofiane
    Bossi, Lionello
    Gonzalez, Anne-Alicia
    Mialhe, Xavier
    Gualda, Emilio J.
    Loza-Alvarez, Pablo
    Blanc-Potard, Anne
    Lutfalla, Georges
    Nguyen-Chi, Mai E.
    ELIFE, 2024, 13
12345