Effect of Heat Stress on Heat Shock Protein 30 (Hsp30) mRNA Expression in Rainbow Trout (Oncorhynchus mykiss)

被引：25

作者：

Liu, Xiaoxia ^{[1
]}

Shi, Haina ^{[1
,2
]}

Liu, Zhe ^{[1
]}

Kang, Yujun ^{[1
]}

Wang, Jianfu ^{[1
]}

Huang, Jinqiang ^{[1
]}

机构：

[1] Gansu Agr Univ, Coll Anim Sci & Technol, 1 Yingmencun, Lanzhou 730070, Gansu, Peoples R China

[2] Qingyang Acad Agr Sci, Qingyang 745000, Gansu, Peoples R China

来源：

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES | 2019年 / 19卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Cold-water species; High temperatures; Small heat shock proteins; Tissue-specific gene expression; ALPHA-B-CRYSTALLIN; GENE-EXPRESSION; CHAPERONE FUNCTION; PLASMA-MEMBRANE; HSP70; GENE; TEMPERATURE; BRAIN; FISH; IDENTIFICATION; HYPERTHERMIA;

D O I：

10.4194/1303-2712-v19_8_06

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Rainbow trout (Oncorhynchus mykiss) is a cold-water species, and often affected by high temperatures. To understand hsp30 mRNA expression trend and molecular mechanism responding to high temperature stress, real-time qPCR was used to quantify hsp30 mRNA expression level in different tissues (heart, liver, gill, brain, spleen and head kidney) in control (18 degrees C) and heat-stressed (25 degrees C for 2, 4, 8 and 12 h) groups. Results showed that hsp30 mRNA in all tissues had already synthesized at 18 degrees C. hsp30 mRNA expression in all tissues was significantly up-regulated and reached the peak after heat stress at 25 degrees C for 2-4 h (P<0.05), then significantly down-regulated at 8 h (P<0.05), and finally returned to control level at 12 h and HSR (P>0.05). The highest hsp30 mRNA expression levels were recorded in heart and liver after 2-4 h. However, the most significant changes of hsp30 mRNA after 2-4 h were observed in gill by 15668.243- and 11452.125-fold compared with 18 degrees C. These results indicated that hsp30 mRNA expression in rainbow trout was induced by heat stress and responded in a time- and tissue-specific manner. Hsp30 can provide some protection in heat stress, but it is only within a certain range.

引用

页码：681 / 688

页数：8

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