CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

被引：26

作者：

Sivanarayana, T. ^{[3
,4
]}

Krishna, Ch. Ravi ^{[3
,4
]}

Prakash, G. Jaya ^{[2
]}

Krishna, K. Murali ^{[2
]}

Madan, K. ^{[2
]}

Rani, B. Sireesha ^{[1
]}

Sudhakar, G. ^{[4
]}

Raju, G. A. Rama ^{[1
]}

机构：

[1] Krishna IVF Clin, Gynecol Div, Visakhapatnam 530002, Andhra Pradesh, India

[2] Krishna IVF Clin, Embryol Res Grp, Visakhapatnam 530002, Andhra Pradesh, India

[3] Krishna IVF Clin, Dept Genet, Visakhapatnam 530002, Andhra Pradesh, India

[4] Andhra Univ, Dept Human Genet, Visakhapatnam 530003, Andhra Pradesh, India

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2012年 / 29卷 / 12期

关键词：

Male infertility; CASA; Motility; Chromatin packing; DNA fragmentation; IN-VITRO FERTILIZATION; DEOXYRIBONUCLEIC-ACID FRAGMENTATION; STRICT CRITERIA; HUMAN-SPERMATOZOA; INFERTILE MEN; ASSISTED REPRODUCTION; INVITRO FERTILIZATION; SEMEN CHARACTERISTICS; FAILED FERTILIZATION; ANILINE BLUE;

D O I：

10.1007/s10815-012-9885-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI. Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively. Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal. Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

引用

页码：1327 / 1334

页数：8

共 57 条

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