Ferritin subunits in CSF are decreased in restless legs syndrome

被引:58
作者
Clardy, SL
Earley, CJ
Allen, RP
Beard, JL
Connor, JR
机构
[1] Penn State Univ, Milton S Hershey Med Ctr, Dept Neurosurg, Hershey, PA 17033 USA
[2] Penn State Univ, Dept Nutr, College Pk, MD USA
[3] Johns Hopkins Sch Med, Dept Neurol, Baltimore, MD USA
来源
JOURNAL OF LABORATORY AND CLINICAL MEDICINE | 2006年 / 147卷 / 02期
关键词
D O I
10.1016/j.lab.2005.06.011
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Restless legs syndrome (RLS) is a neurological disorder that may be related to iron misregulation at the level of the central nervous system. Evidence that iron is involved in RLS comes from magnetic resonance imaging data, autopsy studies, analyses of cerebrospinal fluid (CSF), and correlations of symptoms with serum ferritin. To further examine the possibility that brain iron status is insufficient in RLS, we determined ferritin levels in the CSF. Specifically, we differentiated between the H- and L-subunits of ferritin, because these peptides are expressed from different chromosomes and have different functions. We measured H- and L-ferritin subunit levels in control and RLS human CSF using immunoblot analysis and found that both H- and L-ferritin are significantly decreased in early but not late-onset RLS. Additionally, we quantified total protein in each CSF sample to establish that the decrease in ferritin subunits in RLS did not reflect a decrease in total protein in CSF. Furthermore, we used equal amounts of total CSF protein in the immunoblot analyses, in contrast to previously published studies that provided only volumetric data, to determine which approach was more accurate for quantifying the amount of ferritin relative to other proteins in CSF. Our results establish a protein standard in RLS, provide a comparative analysis of protein-controlled versus volumetric immunoblot techniques, and argue for a profound loss of iron storage capacity in the brain in RLS, specifically in the early onset RLS phenotype. These data suggest that CSF ferritin levels may provide a biomarker for assisting in the diagnosis of RLS.
引用
收藏
页码:67 / 73
页数:7
相关论文
共 63 条
[1]   Evaluating the quality of life of patients with restless legs syndrome [J].
Abetz, L ;
Allen, R ;
Follet, A ;
Washburn, T ;
Earley, C ;
Kirsch, J ;
Knight, H .
CLINICAL THERAPEUTICS, 2004, 26 (06) :925-935
[2]   Family history study of the restless legs syndrome [J].
Allen, R. P. ;
La Buda, M. C. ;
Becker, P. ;
Earley, C. J. .
SLEEP MEDICINE, 2002, 3 :S3-S7
[3]   Restless legs syndrome: diagnostic criteria, special considerations, and epidemiology - A report from the restless legs syndrome diagnosis and epidemiology workshop at the National Institutes of Health [J].
Allen, RP ;
Picchietti, D ;
Hening, WA ;
Trenkwalder, C ;
Walters, AS ;
Montplaisi, J .
SLEEP MEDICINE, 2003, 4 (02) :101-119
[4]  
Allen RP, 2003, SLEEP, V26, pA339
[5]   THE RELATIONSHIP BETWEEN IRON RELEASE, FERRITIN SYNTHESIS AND INTRACELLULAR IRON DISTRIBUTION IN MOUSE PERITONEAL-MACROPHAGES - EVIDENCE FOR A REDUCED LEVEL OF METABOLICALLY AVAILABLE IRON IN ELICITED MACROPHAGES [J].
ALVAREZHERNANDEZ, X ;
FELSTEIN, MV ;
BROCK, JH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 886 (02) :214-222
[6]  
AROSIO P, 1978, J BIOL CHEM, V253, P4451
[7]  
BALLA G, 1992, J BIOL CHEM, V267, P18148
[8]  
BEGUIN Y, 1989, J LAB CLIN MED, V113, P346
[9]   CHARACTERISTICS OF A FERRITIN-BINDING PROTEIN PRESENT IN HUMAN-SERUM [J].
BELLOTTI, V ;
AROSIO, P ;
CAZZOLA, M ;
COZZI, A ;
LEVI, S ;
MELONI, F ;
ZAPPONE, E .
BRITISH JOURNAL OF HAEMATOLOGY, 1987, 65 (04) :489-493
[10]   FERRITIN, TRANSFERRIN, AND IRON IN SELECTED REGIONS OF THE ADULT AND AGED RAT-BRAIN [J].
BENKOVIC, SA ;
CONNOR, JR .
JOURNAL OF COMPARATIVE NEUROLOGY, 1993, 338 (01) :97-113