Microbial composition of defect smear - A problem evolving during foil-prepacked storage of red-smear cheeses

被引:15
作者
Amato, Lea [1 ]
Ritschard, Jasmine S. [2 ]
Kurtz, Olivia [1 ]
Arias-Roth, Emmanuelle [3 ]
Lacroix, Christophe [1 ]
Schuppler, Markus [2 ]
Meile, Leo [1 ]
机构
[1] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Lab Food Biotechnol, CH-8092 Zurich, Switzerland
[2] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Food Microbiol Lab, CH-8092 Zurich, Switzerland
[3] Res Stn Agroscope Liebefeld Posieux ALP, CH-3003 Bern, Switzerland
关键词
GRADIENT GEL-ELECTROPHORESIS; VOLATILE AROMA COMPOUNDS; 16S RIBOSOMAL-RNA; SURFACE MICROFLORA; TEMPORAL TEMPERATURE; PCR; IDENTIFICATION; BACTERIA; GENE; AMPLIFICATION;
D O I
10.1016/j.idairyj.2012.07.012
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
To elucidate the problem of smear defect in foil-prepacked fully-ripened red-smear cheese portions, the microbial composition of 47 smear samples from 2 cheese varieties was analyzed. Cheese variety M was characterized by a diversity of 27 different bacterial species of which 11 represented typical smear microorganisms belonging to Arthrobacter, Brachybacterium, Brevibacterium, Corynebacterium, Microbacterium and Staphylococcus. For variety T 29 different species were identified with 12 isolates representing typical smear microorganisms. Further isolates represented members of Bacillus, Citrobacter, Enterococcus, Facklamia, Morganella, Proteus and Vagococcus. Culture-dependent analysis revealed a stable microbial composition that was confirmed by culture-independent techniques. However, several bacteria with potential to contribute to smear defects by off-flavor or exopolysaccharide production were identified. Our results indicate that the cause of the defect might not be assigned to a single microbial group but rather to the contribution of various microorganisms and their metabolic activity within the foil. (c) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:77 / 85
页数:9
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