Removal of promoter nucleosomes by disassembly rather than sliding in vivo

被引：208

作者：

Boeger, H ^{[1
]}

Griesenbeck, J ^{[1
]}

Strattan, JS ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

MOLECULAR CELL | 2004年 / 14卷 / 05期

关键词：

D O I：

10.1016/j.molcel.2004.05.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work demonstrated the removal of nucleosomes from the PHO5 promoter upon transcriptional activation in yeast. Removal could occur by nucleosome disassembly or by sliding of nucleosomes away from the promoter. We have now activated the PHO5 promoter on chromatin circles following excision from the chromosomal locus. Whereas sliding would conserve the number of nucleosomes on the circle, we found that the number was diminished, demonstrating chromatin remodeling by nucleosome disassembly.

引用

页码：667 / 673

页数：7

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