Association of Pro-apoptotic Bad Gene Expression Changes with Benign Thyroid Nodules

Background/Aim: This study aimed to investigate the role of the mitochondrial apoptotic pathway in benign thyroid nodules. Materials and Methods: Paired samples of nodular and normal tissues were collected from 26 patients with nodular goiters undergoing thyroidectomy. Variable expression of Bcl-2, Bax and Bad genes were evaluated by quantitative PCR. Results: Expression level of Bad gene in nodules was found to be significantly decreased compared to normal tissues p=0.049). A positive correlation was observed between nodule size and Bad expression levels (correlation coefficient=0.563, p=0.004); and this correlation was stronger in hot nodules (n=18, correlation coefficient=0.689, p=0.003). No significant difference was observed between nodular and normal tissue expressions of Bax and Bcl-2. Conclusion: These results suggest that Bad expression correlates with the size of benign thyroid nodules and also its relatively lower expression in nodules, warrant further investigation.