The role of cellular contact and TGF-beta signaling in the activation of the epithelial mesenchymal transition (EMT)

被引：15

作者：

Gasior, Kelsey ^{[1
]}

Wagner, Nikki J. ^{[2
]}

Cores, Jhon ^{[3
]}

Caspar, Rose ^{[2
]}

Wilson, Alyson ^{[4
]}

Bhattacharya, Sudin ^{[5
,6
,7
,8
,9
]}

Hauck, Marlene L. ^{[2
]}

机构：

[1] North Carolina State Univ, Biomath Program, Raleigh, NC 27695 USA

[2] North Carolina State Univ, Coll Vet Med, Raleigh, NC USA

[3] Univ North Carolina & North Carolina State Univ, Joint Dept Biomed Engn, Chapel Hill, NC 27515 USA

[4] North Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA

[5] Michigan State Univ, Dept Biomed Engn, E Lansing, MI 48824 USA

[6] Michigan State Univ, Dept Pharmacol & Toxicol, E Lansing, MI 48824 USA

[7] Michigan State Univ, Ctr Res Ingredient Safety, E Lansing, MI 48824 USA

[8] Michigan State Univ, Inst Quantitat Hlth Sci & Engn, E Lansing, MI 48824 USA

[9] Michigan State Univ, Inst Integrat Toxicol, E Lansing, MI 48824 USA

来源：

CELL ADHESION & MIGRATION | 2019年 / 13卷 / 01期

基金：

美国国家科学基金会;

关键词：

EMT; TGF-; cellular adhesion; epithelial; mesenchymal; breast carcinoma; colon carcinoma; E-CADHERIN EXPRESSION; REPRESSES E-CADHERIN; SLUG; CELLS; SNAIL;

D O I：

10.1080/19336918.2018.1526597

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The epithelial mesenchymal transition (EMT) is one step in the process through which carcinoma cells metastasize by gaining the cellular mobility associated with mesenchymal cells. This work examines the dual influence of the TGF- pathway and intercellular contact on the activation of EMT in colon (SW480) and breast (MCF7) carcinoma cells. While the SW480 population revealed an intermediate state between the epithelial and mesenchymal states, the MC7 cells exhibited highly adhesive behavior. However, for both cell lines, an exogenous TGF- signal and a reduction in cellular confluence can push a subgroup of the population towards the mesenchymal phenotype. Together, these results highlight that, while EMT is induced by the synergy of multiple signals, this activation varies across cell types.

引用

页码：63 / 75

页数：13

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