Rapid High-Specificity microRNA Detection Using a Two-stage Isotachophoresis Assay

被引：48
作者：
Garcia-Schwarz, Giancarlo [1 ]
Santiago, Juan G. [1 ]
机构：
[1] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 44期
基金：
美国国家科学基金会;
关键词：
analytical methods; hybridization assay; isotachophoresis; RNA structures; RNA recognition; EXPRESSION; ENCODES; CANCER;
D O I：
10.1002/anie.201305875
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Focusing in on the small: A two-stage microRNA detection assay uses electrokinetic focusing to speed up hybridization and a functionalized hydrogel for affinity purification. This method detects microRNAs in 15 minutes with single-nucleotide specificity, and processes only 5 ng of total RNA. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：11534 / 11537
页数：4
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