Generation, identification, and functional analysis of monoclonal antibodies against porcine epidemic diarrhea virus nucleocapsid

被引：30

作者：

Yang, Wenting ^{[1
]}

Chen, Wenwen ^{[1
]}

Huang, Jingling ^{[1
]}

Jin, Li ^{[1
]}

Zhou, Yawei ^{[1
]}

Chen, Jianing ^{[1
]}

Zhang, Na ^{[1
]}

Wu, Donglai ^{[2
]}

Sun, Encheng ^{[2
]}

Liu, Guangliang ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, 1 Xujiaping, Lanzhou 730046, Gansu, Peoples R China

[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Heilongjiang, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2019年 / 103卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Porcine epidemic diarrhea virus; Monoclonal antibodies; Nucleocapsid protein; SUBCELLULAR-LOCALIZATION; STRAINS; PROTEIN; CHINA;

D O I：

10.1007/s00253-019-09702-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The variant strains of porcine epidemic diarrhea virus (PEDV) severely threaten the pig industry worldwide and cause up to 100% mortality in suckling piglets. It is critically important and urgent to develop tools for detection of PEDV infection. In this study, we developed six monoclonal antibodies (mAbs) targeting N protein of PEDV and analyzed their applications on enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), western blot assay, and flow cytometry assay. The results demonstrated that all these six mAbs were IgG1 isotype and chain. Among these six mAbs, 3F12 recognizes a linear epitope (VAAVKDALKSLGI) while the other five mAbs recognize different conformational epitopes formed by a specific peptide fragment or the full length of N protein. The functional analysis showed that all these six mAbs were applicable to ELISA, western blot, IFA, and flow cytometry assay. In conclusion, we developed six mAbs against PEDV-N protein to facilitate the early detection of PEDV infection using ELISA, western blot, IFA, and flow cytometry.

引用

页码：3705 / 3714

页数：10

共 30 条

[1] Spatial and temporal epidemiology of porcine epidemic diarrhea (PED) in the Midwest and Southeast regions of the United States [J].