Impact of sperm retrieival on testis and epididymis: an experimental study using Wistar albino rats

被引:7
|
作者
Prithiviraj, Elurnalai [1 ]
Suresh, Sekar [2 ]
Manivannan, Monica [3 ]
Prakash, Seppan [1 ]
机构
[1] Univ Madras, Dept Anat, Dr Arcot Lakshmanasamy Mudaliar Postgrad Inst Bas, Madras 600113, Tamil Nadu, India
[2] Sathyabama Univ, Dent Coll & Hosp, Dept Anat, Madras, Tamil Nadu, India
[3] Saveetha Med Coll & Hosp, Chennai, Tamil Nadu, India
关键词
anti-sperm antibody; apoptosis; histomorphometry; inflammatory reaction; sperm aspiration/retrieval; ANTISPERM AUTOANTIBODY RESPONSE; FINE-NEEDLE-ASPIRATION; MONKEY MACACA-RADIATA; NONOBSTRUCTIVE AZOOSPERMIA; GERM-CELLS; INTRACYTOPLASMIC INJECTION; DEVELOPMENTAL-CHANGES; MUCUNA-PRURIENS; VAS-DEFERENS; FOLLOW-UP;
D O I
10.3109/19396368.2013.809173
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
The aim of this study was to analyze pathophysiological changes after testicular sperm aspiration (TESA) and microsurgical epididymal sperm aspiration (MESA) procedures. Twenty four mature male Wistar albino rats with a proven breeding history, weighing approximately 200-250 gm were used for the study. Animals were randomly divided into four groups (n = 6), i.e., control, sham-control, unilateral TESA, and MESA. Using a 22G needle, the aspiration procedures were done in testis or caudal epididymis. At the end of 60 days of survival, blood samples were collected and processed for antisperm antibody detection by enzyme-linked immunosorbent assay (ELISA). After euthanasia, testes and epididymides were collected and processed for paraffin embedding. Sections were stained with hematoxylin and eosin, and TUNEL technique. Serum antisperm antibody titer significantly increased in TESA (P < 0.001) when compared to MESA. Histomorphometric analysis indicated testicular alterations in TESA and MESA, with significant damage in TESA in both testes (P < 0.001). Following the MESA procedure, ipsilateral caudal and carpus epididymis showed significant alterations (P < 0.001) and no such alterations were seen in the ipsilateral caput and intact contralateral epididymis. TUNEL staining revealed an up-regulation of apoptosis in both contra- and ipsilateral testes of TESA. Needle prick had produced drastic and irreversible alterations in testis of TESA. Ensuing processes of immunological and inflammatory reaction had the potential to disrupt spermatogenesis and increase germ cell apoptosis. However, extrapolating conclusions from the experimental model to the clinic needs to be done cautiously.
引用
收藏
页码:261 / 269
页数:9
相关论文
共 50 条
  • [41] Ameliorative Effect of Naringenin in 5-Fluorouracil-Induced Hepatotoxicity: An Experimental Study on Albino Wistar Rats
    Vemula, Sravathi
    Mylaram, Jeevanalatha
    Yadala, Ravikumar
    Banothu, Anilkumar
    Aasritha, Sanduri
    Mounika, Kamishetti
    Hanuman, Donga Durga Veera
    INDIAN JOURNAL OF ANIMAL RESEARCH, 2024, 58 (08) : 1294 - 1302
  • [42] The effects of tamoxifen on radiation-induced pulmonary fibrosis in wistar albino rats: results of an experimental study
    Bese, NS
    Umay, C
    Ilvan, S
    Salar, S
    Altug, T
    Ober, A
    RADIOTHERAPY AND ONCOLOGY, 2004, 73 : S278 - S278
  • [43] The effects of tamoxifen on radiation-induced pulmonary fibrosis in Wistar albino rats: Results of an experimental study
    Bese, Nuran Senel
    Umay, Cenk
    Yildirim, Sukru
    Ilvan, Sennur
    Dirican, Ahmet
    Salar, Seda
    Altug, Tuncay
    Ober, Ahmet
    BREAST, 2006, 15 (03): : 456 - 460
  • [44] Adverse Effects of Hydroxychloroquine on Testicular Tissue and Sperm Quality: An Experimental study on Male Albino Rats
    Mukhopadhyay, Sagarika
    Samanta, Tanushree
    Khanra, Suman Kalyan
    Jana, Anup
    JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2024, 18 (07) : CC10 - CC14
  • [45] Amelioration of radiation-induced lung injury by halofuginone: An experimental study in Wistar-Albino rats
    Calik, M.
    Yavas, G.
    Calik, S. G.
    Yavas, C.
    Celik, Z. E.
    Sargon, M. F.
    Esme, H.
    HUMAN & EXPERIMENTAL TOXICOLOGY, 2017, 36 (06) : 638 - 647
  • [46] Effect of Perilla frutescens Fixed Oil on Experimental Esophagitis in Albino Wistar Rats
    Arya, Ekta
    Saha, Sudipta
    Saraf, Shubhini A.
    Kaithwas, Gaurav
    BIOMED RESEARCH INTERNATIONAL, 2013, 2013
  • [47] Ethonalic leaf extract of Andrographis paniculata-induced oxidative stress in the testis and epididymis of Male Wistar Rats
    Narayana, Satya K.
    Naidu, Kumaraswamy C.
    Trinath, D.
    Reddy, Sreenivasula P.
    Reddy, Srinivasulu M.
    Suneetha, Y.
    MEDICAL SCIENCE, 2014, 14-15 (54-62) : 7 - 13
  • [48] Antioxidant action of alpha lipoic acid on the testis and epididymis of diabetic rats: morphological, sperm and immunohistochemical evaluation
    Neves Silva Correa, Lanna Beatriz
    Soares da Costa, Carlos Alberto
    Ribas, Jose Antonio Silva
    Boaventura, Gilson Teles
    Chagas, Mauricio Alves
    INTERNATIONAL BRAZ J UROL, 2019, 45 (04): : 815 - 824
  • [49] Influence of acetazolamide on AQP1 gene expression in testis and on sperm count/motility in epididymis of rats
    Yu, HM
    Sun, BM
    Bai, Q
    Koide, SS
    Li, XJ
    ARCHIVES OF ANDROLOGY, 2002, 48 (04): : 281 - 294
  • [50] Impact on behavioral changes due to chronic use of sertraline in wistar albino rats
    Mukherjee, Shatavisa
    Sen, Sukanta
    Biswas, Arunava
    Barman, Tapan Kumar
    Tripathi, Santanu Kumar
    INDIAN JOURNAL OF PHARMACOLOGY, 2014, 46 : S2 - S2