MgrA Activates Expression of Capsule Genes, but Not the α-Toxin Gene in Experimental Staphylococcus aureus Endocarditis

被引:37
作者
Gupta, Ravi Kr [1 ]
Alba, Jimena [1 ]
Xiong, Yan Q. [2 ]
Bayer, Arnold S. [2 ]
Lee, Chia Y. [1 ]
机构
[1] Univ Arkansas Med Sci, Dept Microbiol & Immunol, Little Rock, AR 72205 USA
[2] Univ Calif Los Angeles, Harbor Med Ctr, Los Angeles Biomed Res Inst, Torrance, CA 90509 USA
关键词
Staphylococcus aureus; virulence; infective endocarditis model; mgrA; capsule; alpha-toxin; IN-VITRO; GLOBAL REGULATOR; MICROCAPSULE EXPRESSION; VIRULENCE DETERMINANTS; SEPTIC ARTHRITIS; TYPE-5; CAPSULE; AGR; SARA; SAE; POLYSACCHARIDE;
D O I
10.1093/infdis/jit367
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Staphylococcus aureus produces numerous virulence factors but little is known about their in vivo regulation during an infection. Methods. The production of capsule and alpha-toxin, and the expression of their respective genes, cap5 and hla, were analyzed by comparing CYL11481 (derivative of Newman) and its isogenic regulatory mutants in vitro. The temporal expression of cap5 and hla and the regulatory genes in vivo was carried out using a rat infective endocarditis model. Results. In vitro analyses showed that capsule was positively regulated by MgrA, Agr, Sae, ArlR, and ClpC, and negatively by CodY and SbcDC. The alpha-toxin was positively regulated by MgrA, Agr, Sae, ArlR, and SbcDC but negatively by ClpC and CodY. In vivo analyses showed that cap5 expression correlated best with mgrA expression, whereas hla expression correlated best with sae expression. Mutation in mgrA drastically reduced cap5 expression in vivo. Conclusions. Our results suggest that, in vitro, Agr is the most important regulator for capsule and alpha-toxin production, as well as for cap5 transcription, but SaeR is the most critical for hla transcription. However, in vivo, MgrA is the major transcriptional regulator of capsule, but not alpha-toxin, whereas saeR expression correlates best with hla expression.
引用
收藏
页码:1841 / 1848
页数:8
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