Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometry method for determination of isoniazid in human plasma

被引：33

作者：

Huang, Liusheng ^{[1
]}

Marzan, Florence ^{[1
]}

Jayewardene, Anura L. ^{[1
]}

Lizak, Patricia S. ^{[1
]}

Li, Xiaohua ^{[2
]}

Aweeka, Francesca T. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Sch Pharm, Dept Clin Pharm, San Francisco, CA 94110 USA

[2] Univ Calif San Francisco, Sch Pharm, Dept Biopharmaceut Sci, San Francisco, CA 94110 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 03期

关键词：

LC-MS/MS; Isoniazid; Nialamide; Human plasma; Method development; Silica column; EXTRACTION; QUANTIFICATION; TUBERCULOSIS; PYRAZINAMIDE; RIFAMPICIN;

D O I：

10.1016/j.jchromb.2008.12.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An LC-MS/MS method for the determination of isoniazid in human plasma was developed and validated. Human plasma aliquots of 100 mu L were used for analysis. The assay used nialamide as the internal standard. The calibration curve concentration range was 50-10,000ng/mL Sample preparation utilized protein precipitation, and the supernatant was directly injected onto silica column without reconstitution. The recovery was over 90% and matrix effect was negligible. The method is simple and fast, which is advantageous in respect to instability of isoniazid in human plasma and loss on reconstitution due to its low molecular weight. Published by Elsevier B.V.

引用

页码：285 / 290

页数：6

共 11 条

[1] A liquid chromatography/tandem mass spectrometry method for the simultaneous quantification of isoniazid and ethambutol in human plasma [J].

Chen, XY ;

Song, B ;

Jiang, HJ ;

Yu, K ;

Zhong, DF .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (18) :2591-2596

[2] Sensitive liquid chromatographic technique to measure isoniazid in alveolar cells, bronchoalveolar lavage and plasma in HIV-infected patients [J].

Delahunty, T ;

Lee, B ;

Conte, JE .

JOURNAL OF CHROMATOGRAPHY B, 1998, 705 (02) :323-329

[3]

*FDA, 2005, ACTG GUID METH DEV V

[4]

GROSSET JH, 1989, REV INFECT DIS, V11, pS347

[5] Direct injection of solid-phase extraction eluents onto silica columns for the analysis of polar compounds isoniazid and cetirizine in plasma using hydrophilic interaction chromatography with tandem mass spectrometry [J].

Li, AC ;

Junga, H ;

Shou, WZ ;

Bryant, MS ;

Jiang, XY ;

Weng, ND .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (19) :2343-2350

[6] Quantification of isoniazid and acetylisoniazid in rat plasma and alveolar macrophages by liquid chromatography-tandem mass spectrometry with on-line extraction [J].

Ng, Ka-yun ;

Zhou, Huiyu ;

Zhang, Yan Ling ;

Hybertson, Brooks ;

Randolph, Theodore ;

Christians, Uwe .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 847 (02) :188-198

[7]

OLSON WA, 1977, CLIN CHEM, V23, P745

[8]

Smith PJ, 1999, INT J TUBERC LUNG D, V3, pS325

[9] Simultaneous determination of first-line anti-tuberculosis drugs and their major metabolic ratios by liquid chromatography/tandem mass spectrometry [J].

Song, Sang Hoon ;

Jun, Sun Hee ;

Park, Kyoung Un ;

Yoon, Yeomin ;

Lee, Jae Ho ;

Kim, Jin Q. ;

Song, Junghan .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (07) :1331-1338

[10] Therapeutic monitoring of isoniazid, pyrazinamide and rifampicin in tuberculosis patients using LC [J].

Unsalan, S ;

Sancar, M ;

Bekce, B ;

Clark, PM ;

Karagoz, T ;

Izzettin, FV ;

Rollas, S .

CHROMATOGRAPHIA, 2005, 61 (11-12) :595-598

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