Enabling Quantitative Analysis in Ambient Ionization Mass Spectrometry: Internal Standard Coated Capillary Samplers

被引:32
作者
Liu, Jiangjiang [1 ]
Cooks, R. Graham [2 ]
Ouyang, Zheng [1 ]
机构
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
DESORPTION ELECTROSPRAY-IONIZATION; PAPER-SPRAY; THERAPEUTIC DRUGS;
D O I
10.1021/ac401056q
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We describe a sampling method using glass capillaries for quantitative analysis of trace analytes in small volumes of complex mixtures (similar to 1 mu L) using ambient ionization mass spectrometry. The internal surface of a sampling glass capillary was coated with internal standard then used to draw liquid sample and so transfer both the analyte and internal standard in a single fixed volume onto a substrate for analysis. The internal standard was automatically mixed into the sample during this process and the volumes of the internal standard solution and sample are both fixed by the capillary volume. Precision in quantitation is insensitive to variations in length of the capillary, making the preparation of the sampling capillary simple and providing a robust sampling protocol. Significant improvements in quantitation accuracy were obtained for analysis of 1 mu L samples using various ambient ionization methods.
引用
收藏
页码:5632 / 5636
页数:5
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